Why does my MTT Assay remain in purple color and does not change to yellow?

19 January 2021 5,098 4 View

staircase ramp using VPWL in cadence ?

how to generate staircase ramp using VPWL signal in cadence ?

27 December 2020 1,521 3 View

In the absence of laboratory test or field data, how can we determine the value of coefficient of consolidation for analytical methods?

My research involves comparing the results of finite element analysis ( using ABAQUS) with analytical results. The problem is drain induced consolidation in embankments.

15 September 2018 1,254 3 View

How to select the order of an autoregressive model?

20 July 2016 5,502 7 View

What are the possible impacts of implementing multidimensional information modelling in construction ?

08 May 2016 5,507 1 View

How do I connect a 2 Wire strain gauge to QuantumX MX1615B without adding temperature compensation?

I need to connect an HBM K-LY 4/120 strain gauge(Linear 2 wire) to HBM QuantumX MX1615B DAQ system. This will be mainly done for the calibration of a custom built amplifier circuit. Although...

27 December 2015 8,014 2 View

What are the prospects of Passion fruit seed oil?

Recently I've extracted passion fruit seed oil after studying about its recovery from seed. Now i wanna know if there is any edible or medicinal purpose for it. Looking forward for your answers

25 December 2015 4,076 3 View

Does there exist periodic Gaussian function?

Is there any way of producing periodic Gaussian pulses such that the bell shape repeats itself with a defined period. Does there exist any solution for some differential equation which resembles...

26 May 2015 2,986 13 View

PFGE- Why is there a smear instead of separate bands?

I am doing PFGE and standardizing it with pulse marker. Instrument is new from biorad CHEF DRIII. I am following the protocol provided by biorad but not getting the expected result with pulse...

04 June 2014 575 17 View

Why should additional NaCl be added for nuclear protein extraction (nfkb isolation)?

I am trying to isolate a nuclear protein NF-kB from raw cells in order to do a western blot. I have searched some protocol for nuclear isolation and finally came up with one. But in that...

11 April 2014 2,153 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View