I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?
Malaria is the highest killer disease in Africa, yet no vaccine has been developed against it. Any hope in sight?
05 October 2020 5,670 7 View
I downloaded a components.cif file and filled the location of my .pdb files as shown in the picture below. It still showed error. The program usually opens but doesn't display the pdb files I want...
25 May 2020 8,509 2 View
By the way I also need advice on which correlations to extract from factor studies ( exploratory, confirmatory, & SEM PLS) for meta analysis?
16 March 2020 7,217 3 View
there is a name called Kadimu bay in Siaya, kenya. However, there is also a historical figure called Dimu who seems to be related to the Luo. What is the link between the Egypt Dimu and the Kenya...
29 January 2020 5,176 1 View
When meta analyses on the same topic yield different results with no consensus, can one conduct a meta analysis on the Meta analytic studies?
19 December 2019 6,113 4 View
I intend to run a sequential mediation for a study but am at a lost as to my data. My search shows process macro is not for panel data, but my data is panel. can i be assisted in running a...
28 May 2019 8,256 20 View
I desire to know the contributive effect of the solvent, white spirit oon the drying time of oil-based paints made from alkyd resiin
07 January 2019 5,695 1 View
The goal here is to provide my students with a relatively easy program to use for computing effect size estimates for DIF [item-level invariance] within IRT (parametric). Xcalibre 4.2 offers this...
20 June 2015 3,702 2 View
while calculating PD from which passage number should we take into consideration? Passage 0 (culture initiation) will not have the absolute MSC cell count since we just add Mononuclear cells from...
05 April 2015 8,561 5 View
Does culture passaging or media change reduce endotoxin levels?
29 December 2014 9,274 12 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...
25 February 2021 1,011 3 View