I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?
How can one frame a set of questions to know if a particular group of persons have access to computers and mobile devices.
01 May 2024 5,331 0 View
Hello intellectuals people out there. i want to formulate 5 dietary treatment for rabbits using sweet potatoe peel meal as replacement for maize. how can i balance the 5 different treatment to...
06 December 2023 5,472 0 View
Hi, I need a rough idea of the time between egg laying and first feeding (hatching? Gosner stage ca 32) of the common spadefoot toad. I need also to know roughly at which temperature the hatch...
29 April 2023 4,850 0 View
Knowing researchers who read my papers will help me establish connections, exchange ideas, and collaborate on future research. I particularly need their feedback to improve my research and...
27 April 2023 4,163 1 View
Could anyone who has published a book with Nova Science Publishers advise on their experience with the publisher?
10 March 2022 7,894 4 View
I am interested to acquire Z-stacks via brightfield microscopy of spheroids comprised of Head and Neck cancer cells. My objective is to acquire sphericity and volume on day 0 of the experiment...
17 August 2021 2,251 6 View
Please I want a questionnaire for socioeconomic status affecting academic achievements
09 August 2021 754 4 View
I did some work on MOE and saved my work as .mol2 files and now, I wish to convert them to SMILES files but am really having difficulties. I will be really glad if anyone could be of help.
08 June 2021 9,661 9 View
I did docking and this was a question that has really been in my mind and after searching, I still did not have an answer which is really satisfactory. Please I will be really grateful if anyone...
04 June 2021 3,387 5 View
I downloaded a protein from the PDB and realized it was bound to two native ligands. So, I wish to know why the protein was bound to the two native ligands and not one.
10 May 2021 5,496 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View