I was wondering if anyone has experience or has worked with this test in their research?

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How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What is the reason of stacking DNA in agarose gel well?

we electrophoresis PCR product on 0.7% agarose gel at 120V for 1 hour, our target size is 5000 bp but our DNA stack at wells and do not com in to gelldo you have any suggestion for this?

02 July 2024 5,235 0 View

Is it possible to increase the intensity of UV-vis absorption of DNA EITH A LIGAND IN the groove BINDING mode?

Is it possible to increase the intensity of UV-vis absorption in the interaction of the groove connection mode?

29 June 2024 4,867 4 View

Hello, I wanted to know if it is a necessity to perform MTT test in three time points to evaluate the cytotoxicity of a metal alloy?

I am working on a magnesium based alloy, and i wanted to evaluate the cytotoxicity of my alloy with MTT assay. I want to know the reason why most of the researchers perform the MTT assay in three...

28 June 2024 2,570 1 View

Population coverage analysis for some MHC II alleles?

Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...

20 June 2024 2,155 1 View

Separating Wind Wave and Swell Parameters in Wavewatch III Model?

Hello, I am using the Wavewatch III model for my research . Could anyone clarify if it's possible to extract wind wave and swell parameters separately from this model?

12 June 2024 3,269 4 View

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

10 June 2024 9,523 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How do I western blot of serum proteins without any unspecific binding of secondary antibody to my blot?

Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...

03 June 2024 5,993 8 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

What is the difference between neuropsychology and cognitive neuroscience ?

The main differences

14 July 2024 3,158 4 View

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

06 June 2024 2,234 1 View

Internationally recognised publishing outlets specialised in publishing science-based futuristic papers?

Does anyone use or know of any internationally recognised and reliable publishing outlet (i.e. Journals) that are specialised for submission of "futuristic" articles which are based on the...

28 May 2024 2,946 2 View

How can we effectively model RPE signals at the single-trial level in EEG data?How can researchers disentangle overlapping ERP components effectively?

Dear all, I need your expertise on this matter. I have an article under review and I got some comments that I need your help with: In my research, I investigated the FRN (200_300ms post_feedback...

23 May 2024 4,953 0 View

Can anyone explain why my EGFP expression increased (but Ai9 RFP decreased -like normal) after imaging with a confocal microscope?

I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...

23 May 2024 7,776 2 View

Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

12 May 2024 4,614 3 View

Should a biphasic electrical stimulation pulse on neural networks be positive followed by a negative phase or negative followed by positive phase?

I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...

09 May 2024 7,167 1 View