How do we calculate Limit of detection (LOD) and limit of quantification (LOQ) of ddPCR??

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Caroline Weydert

Hi, there are multiple guidelines and recommendations available for this:

-A good start might be this article: Article Methods to determine limit of detection and limit of quantif...

based on CLSI definitions.

- ISO 20395 describes also LOD, LOQ etc

There are also application-specific guidelines (pharmacopeia, bioanalysis Article Recommendations for Method Development and Validation of qPC...

, ....)

Mousumi Mary

Thanks