Can anyone please provide me a detailed protocol to FACS out astrocytes and neurons from brain tissue? Also how much minimum amount of tiisue is needed to get proper count of astrocytes and neurons
Dear all, We've been doing transesterification with our raw materials - a mixture of pairing oil (which is said to came from the skin part of a coconut) and crude coconut oil. During the addition...
14 July 2024 2,200 0 View
What is the specific cucumber (Cucumis sativus) biomarker for standerdization and fingerprinting using HPLC.
01 June 2024 1,926 0 View
Hybrid composite matrix -Aluminium Alloy Reinforcement - Egg shell and SiC
29 March 2024 5,649 6 View
I am making compounds for SAR, to make the compounds cell permeable I am introducing, F and CF3 , WHAT else funtionality can be introduced which have the same effect as -CF3. Please give some...
15 January 2024 1,181 2 View
My compound is soluble in DMA, but even a drop of water causes it to precipitate out. I want to administer it in mice for in-vivo study through intra-peritoneal injection route. Which vehicle...
15 January 2024 4,050 0 View
I would like some guidance and what to look for and if this is too broad, how to narrow it down. I am a grad student in social work and this is my capstone project thank you
31 August 2023 1,672 1 View
As an antioxidant role of zinc, conflicting reports are pouring. It is recognized that Zn has prominent role in reducing oxidative stress by scavenging and decreasing production of ROS. On the...
19 June 2023 9,769 1 View
I already calculated the activation energy from the output file generated from gaussian. However, I am having a hard time looking for reference material to compare to. My system is CH3Cl + OH-...
27 May 2023 7,673 1 View
Hi, I conducted SN2 reaction transition state determination in gaussian. I calculated the activation energy of the reaction by using the free energy I obtained in the output file. (Free energy...
25 May 2023 5,207 1 View
I am conducting a study on transition state. In order to determine the activation energy, I will use the formula ∆G = ∆H -T∆S And in order to solve for ∆H, I use the formula H = E + ZPE + RT...
07 May 2023 4,083 4 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View