Biotechnological Techniques

To what extent is it advisable to use commercially available competent cells for transformation?

How much efficiency will remain in commercially available competent cells after one month?

05 December 2013 6,170 12 View

How to extract plasmids from bifidobacterium?

I want to extract plasmids from bifidobacterium. I have tried to ground the cells in liquid nitrogen, but still failed. Could anybody tell me another method? Thank you very much.

10 October 2013 7,939 1 View

Can anyone tell me what will happen if you keep your protein precipitated in Methanol/0.1MAmmonium acetate for more than a week?

Isolation of protein from plant leaves.

01 October 2013 4,263 1 View

Does putting petries under the UV (for 15 min before inoculation) change the composition of media?

The media is composed of nutrient agar, ammonium sulfate, sodium acetate, nickel chloride which its pH adjusted to 9 with NaOH.

24 September 2013 9,789 12 View

problem with shRNA ligation

I've been trying to ligate a shRNA fragment into pCAMBIA 1305.1 plasmid. The fragment was ordered from a company in the US. As they provide annealing service, I asked them to anneal my oligos. I...

25 June 2013 9,264 7 View

Can anybody suggest me the concentration of ammonia needed to be used for pH control in fed batch fermentation?

I am working on production of acid metabolites in E.coli in which I have to compare the effect of various bases like NaOH, KOH and Ammonia etc. Could anybody please suggest the concentration of...

19 June 2013 2,107 13 View

Looking for a good protocol or reference to elute protein from native-PAGE gel.

How can I elute protein from page gel? Can anyone suggest a protocol or reference.

30 May 2013 7,573 4 View

Protocol for Nitrilase Immobilization.

Does anyone know which is the best method for nitrilase immobilization in order to preserve enzymatic activity?

20 April 2013 7,271 2 View

How to determine fertilized egg from unfertilized egg in oviparous species like hen or ostrich? Is it possible now to detect?

Determination of fertilized eggs is commercially important. For example, in ostrich poultry the benefit of detecting fertilized ostrich eggs allows you to keep them for a generation and sell rest...

15 March 2013 300 7 View

In which ratio/concentration I should add IPTG+X-gal to ger blu-white colonies?

I tried to prepare blue-white colonies to check the tranformation efficiency, but coldn't get enough observation with my E.coli plates. I added IPTG and X-gal into a vial and then aliquot it and...

26 February 2013 5,281 2 View

Has anyone worked with ThermoScientific QuantaRed ELISA substrate?

I am trying to measure activity of anti-myc scFv expressed in fungi. I use cell extracts for the ELISA. I have problems in measuring the fluorescence. The readings I get are seem strange. The...

22 February 2013 8,002 1 View

Can anyone suggest a leader peptide sequence for efficient secretion of a recombinant protein, transiently expressed in 293 cells?

Secreted recombinant transient protein expression

20 February 2013 3,457 13 View

Can we use a bacteriophage containing product to kill or remove potentially pathogenic bacteria from water?

If we have a product which contains bacteriophages which specifically kill pathogenic bacteria in contaminated water, will it work efficiently? Will people use this product for water disinfection?

18 February 2013 5,440 1 View

Has anybody in India used GeneScript for the synthesis of their gene of interest?

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11 February 2013 6,124 5 View

SDS-electrophoresis of protein samples containing 0,5% of Triton X-100.

I have a problem with PAGE SDS-electrophoresis of protein samples containing 0,5% of Triton X-100. Samples travel wery poorly and can't be properly stained (with Coomasie or silver - I get just...

22 January 2013 9,570 3 View

How to separate unknown antibiotics by using thin layer chromatography?

antibiotics from Marine actinobacteria

17 January 2013 2,471 1 View

How are Silicia Spin Columns made and are there types?

I'm curious if anyone knows how spin columns are made. I can't seem to find any technical articles or instructions so I'm at a loss. Additionally, I'm interested in knowing if anyone has any idea...

01 January 1970 4,357 3 View

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