In which ratio/concentration I should add IPTG+X-gal to ger blu-white colonies?

More Umapriyatharshini Rajagopalan's questions See All

What could be some reasons why unstimulated monocytes are getting activated?

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How to increase the adhesion of electrospinned nano fiber's?

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Regarding COMSOL simple solid mechanics: Please help me out?

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Can I measure the Work Function of a thin film for amorphous material using XPS?

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24 May 2020 7,637 15 View

Why should I join some research society?

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12 February 2020 5,358 4 View

What is the maximum RMS current for GEIS that I can use for testing commercial 18650 batteries of capacities from 1 Ah to 3.3 Ah?

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Under which conditions poling depletes the performance of Piezoelectric material?

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Can somebody thorow some light on this configuration of PE loop??

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The mixture was precipitated in methanol to get powder? what does it mean

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17 February 2015 5,813 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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Unknown debris sheets observed in cell culture flasks?

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Koen Venken

IPTG is in water. X gal is in DMSO or DMF.

You should add one to the plate, spread, and then add the other one, and spread again. Then add the bugs and spread.

Umapriyatharshini Rajagopalan

Thanks alot Minde and Koen Venken. Those so helpful to me, coz I have tried several times but yet could not make it. Hope I can make this time with this methodology.