Has anybody in India used GeneScript for the synthesis of their gene of interest?

More Nadim Ahamad's questions See All

How the following depth image parameter are derived?? in attachemnt?

how the following depth image parameter are derived?? in attachemnt

27 July 2023 2,465 0 View

How to model a query protein having 3036 residues?

I need to model a protein having 3036 residues and it doesn't have any good template. I tried I-Tasser, Robetta, tr-ROSETTA and they have a limit of 1200 residues. I also tried AlphaFold colab...

15 June 2022 6,682 1 View

How to make pandas data-frame from a mat file which contains data as a dictionary?

I have a data set in .mat file. I need to represent it as a pandas data-frame in python. I have followed all the solutions available on Stack Overflow and any other website but those did not...

12 August 2021 4,304 3 View

How to get the final structure of a protein-ligand after performing 100ns MD simulation using GROMACS?

I have performed a 100ns simulation of a protein-ligand complex. Now I want to get the final structure of the complex after simulation and superimpose it with the initial structure. What is the...

31 March 2021 8,541 4 View

How to decrease use of CPU cores and GPU percentage in GROMACS?

During any simulation of complexes using GROMACS utilizing all the 12 cores and around 90% of GPU, my pc GPU temperature reaches up to 85 degree celcius. Is there any way to decrease the...

26 March 2021 8,768 2 View

How to restart simulation in Gromacs?

I tried to run a 500ns MD simulation of a protein in Gromacs. Due to power failure, my pc turned of after around 450+ ns. Now I want to restart the simulation from that point. I used the following...

13 November 2020 9,410 3 View

How to calculate RMSF per residue name vs time in Gromacs?

I have done 100ns simulation using Gromacs. To calculate the RMSF, I used the following command- gmx rmsf -f md_noPBC.xtc -s md.tpr -o rmsf.xvg -res But when I plotted the graph in excel, I found...

12 November 2020 4,904 2 View

What kind of research can be explored in the gig economy respectively in tax matters?

I am looking for any opportunity to explore any tax issues/matters relating to the gig economy. Perhaps, some of you might be giving some thoughts here. Thanks in advance.

19 October 2020 9,951 5 View

How to transfer CST designed antenna to Adobe Illustrator software?

The microstrip antenna was designed in CST Studio Suite and should be transferred in to Adobe Illustrator design to prepare for stencil screen preparation

05 October 2020 6,571 2 View

Is there any tissue-engineered synthetic/cell-seeded implant approved for Corneal or Retinal regeneration?

How are the patients with severe damage in corneal or retinal surfaces being treated currently? As far as I understand, for such implantation the shortage of donor, poor graft survival and...

19 June 2020 2,389 2 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Ternary in Electrical Engineering?

Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...

28 July 2024 5,771 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

Andrei I. Pozniakovsky

Yes. Satisfied. Reasonable price, accurate and fast enough service.

Oops, sorry. I'm not in India. :-)

Nadim Ahamad

Ohh.. thats fine..:)

John Laudenslager

Yup. Fine job, reasonably quick and inexpensive. Now using Life Technologies (Invitrogen) because they are local for us.

Ya I also tried for that ...but they had their own limitations to deal with pathogen's stuff. Anyway, they are not local for 'us' but for US....:)