Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

TCE Stock solution concentration for SDS-PAGE?

Greetings, I'm trying to standarize TCE free staining method to visualize protein bands over SDS-PAGE, it's never been used in our lab. I've seen many published papers using this method, and the...

17 August 2020 6,620 3 View

No title

Good morning, dear community, I have a preliminary report from an in silico analysis of non-coding short sequences against the SARS-CoV-2 virus. I'd like some advice about which indexed journals...

18 July 2020 5,365 1 View

Mask for outdoor exercise during covid pandemics?

Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...

08 July 2020 4,927 2 View

Help editing citantion style in Mendeley. Multiple paper by the same authors?

I'm trying to edit an existing citation style to suit my needs (Journal of Tropical Ecology). I edited it so the inline citations go from newest to oldest reference (Smith et al 2019, Jones et al...

15 June 2020 9,926 4 View

How to measure ratio/amount of PCR products?

Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure...

02 February 2020 7,599 3 View

Why GFP PCR in E. Coli is not about 50% 50% with respect of GFP expressing bacteria and non-GFP expresing bacteria?

As you can see, most of our colonies express GFP while we expected to get only 50% of these or less.

04 December 2017 8,378 3 View

Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

05 November 2017 5,899 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View