Waters ACQUITY UPLC - Issues with PDA?

I'm attempting to use a Waters Acquity UPLC prior to mass spec and I'm seeing really weird chromatograms. When I inject water, the PDA detector shows a steadily increasing signal (looks like a...

01 April 2020 4,117 2 View

Troubleshooting site directed mutagenesis of a large (10kb) plasmid?

Hi, I'm working on introducing single point mutations with mostly overlapping primers I designed using the QuickChange XL manual, and doing the PCR with phusion polymerase. My plasmid is quite...

26 July 2018 6,903 3 View

Is anyone aware of data on wealth distributions in different countries?

Hello, I am looking for data on distribution of wealth/net worth within countries around the world. Something like a frequency distribution of net worth within a population, that would allow me to...

17 February 2018 2,439 4 View

What is a good parent questionnaire measure of children's/adolescent's stressful life events?

For study planning purposes, to be used with typically developing youth :)

03 August 2017 5,607 2 View

Marker, antibody for submucosal immune cells in gut tissue?

I am trying to identify submucosal immune cells in samples of rat gut tissue via IHC, but need a good marker/antibody for these cells. Does anyone have any suggestions as to which marker may be...

28 July 2016 8,314 1 View

RBC Lysis Problems with Neutrophil Isolation from Rat Whole Blood?

15 June 2016 354 5 View

ACK Lysis buffer - RT vs cold?

I'm trying to use ACK lysis buffer to lyse RBCs from whole blood samples after an initial lysing with H2O (restoring isotonicity with 10% NaCl). My last step is using RIPA buffer + protease...

07 April 2016 3,725 1 View

Can anyone suggest some R script for repeated measures GLM using the lme4 package?

31 August 2014 3,024 8 View

How do you use the MAKESENS 1.0 Excel Template without access to an earlier version of Excel?

I struck a goldmine coming across this spreadsheet template, and I am trying to use it for my research. However, the macros aren't functional in compatibility mode, and I can't even begin to...

01 January 1970 232 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View