Can we consider hemagglutination assay for dengue virus as a quantitative method???

For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.

04 July 2019 3,881 1 View

How different is western blot protocol for phosphorylated protein from regular western blot?

30 August 2017 2,594 9 View

Can anyone give me a protocol for stable luciferase transfection of murine cell lines?

I want to do a stable luciferase transfection of murine PDAC (PANC02) and colorectal (CT26) cell lines. Please help me find where to obtain the protocol and reagents. Thank you. Sincerely, Lakshmi.

05 April 2017 2,462 4 View

Whats is the maximum concentration of NaOH that is not toxic to cell cultures?

18 September 2016 998 6 View

If anyone has done GCMS for tetrodotoxin detection?

If anyone has done GCMS for tetrodotoxin detection, please help me to know the compound names for the characteristic fragments of 2-amino 6-hydroxymethyl 8 hydroxyquinazoline that forms peaks at...

02 September 2016 3,180 2 View

I just want to know factors affecting crystallite size of a materials?

01 April 2016 1,566 13 View

Can someone suggest protocols for assaying catalase, glutathione peroxidase, superoxide dismutase and lipid peroxidase activities in animal tissues?

18 September 2014 2,279 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View