I want to extract plasmids from bifidobacterium. I have tried to ground the cells in liquid nitrogen, but still failed. Could anybody tell me another method? Thank you very much.
Hello, see this article.
http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.1997.00059.x/abstract
The articles from Mitsuoka T. are so good in general.
Best regards,
L.
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