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Questions related from Mac Dz
Which culture media is best for disk diffusion antibacterial susceptibility testing? Agar Bacteriological (Agar No. 1, Oxoid) or Müller-Hinton agar?
03 March 2020 4,403 3 View
TC = Tissue-Culture treated
02 February 2020 3,351 6 View
Which one kills bacteria & fungi faster & more effectively?
02 February 2020 1,452 5 View
Pre-diffusion of agar plates seeded with bacteria and inoculated disks for disk diffusion assay.
02 February 2020 657 12 View
Has anyone observed a quality variation during antibacterial susceptibility testing like in disk diffusion assay? Oxoid™ vs Other brands?
02 February 2020 5,476 3 View
How to prepare 50% DMSO from 100% DMSO? What is the solution should I use to dilute it? I have water and chloroform. The plant material is initially extracted in chloroform. Need to...
12 December 2019 9,766 8 View
How long should it be good to use for antimicrobial activity testing? If the extracts were kept at room temperature (28°C ) & in the refrigerator 4°C?
12 December 2019 9,936 10 View
Can I make 100 ml of Müller-Hinton agar from 38 g mixed in 1 L Müller-Hinton agar solution?
10 October 2019 6,116 7 View
Which is a better medium for preparing 0.5 McFarland turbidity standard for antibacterial activity assay? saline or Müller-Hinton broth?
08 August 2019 6,096 21 View
What would be the concentration of a 10μl aliquot taken from a 10ml solution with a concentration of 1000mg/ml?
08 August 2019 3,791 6 View
What are the chances of increasing IELTS band score for writing & speaking by 0.5 after a remark (EOR)?
05 May 2019 6,989 12 View
Which plant extract is better for antimicrobial sensitivity testing (disk diffusion assay)? centrifuged or non-centrifuged extract?
02 February 2019 1,826 8 View
The patient who was 63 years old was hospitalized with a pulse rate of 35 bpm with irregular heart rhythm on ECG and had breathing difficulty with severe fatigue. She was diagnosed with a third...
08 August 2018 7,761 27 View
Low lymphocyte count High monocyte count High RBC count Low MCHC (mean corpuscular hemoglobin concentration) High serum creatinine High C-reactive protein High erythrocyte sedimentation rate...
05 May 2018 8,930 10 View
Any risk of contamination? In case of antibiotic sensitivity testing?
02 February 2018 4,712 28 View
Maceration of plant material in distilled water at room temperature (30°C) for 24 hours? or Maceration of plant material in distilled water at 4°C for 24 hours?
01 January 2018 2,977 8 View
What is the difference between the plant species Bunchosia armeniaca and Bunchosia glandulifera? Please do post pictures if you have.
01 January 2018 5,286 10 View
Multiple publication of findings related to bioactivities of the same plant species?
12 December 2017 8,161 8 View
25°C? 28°C? 30°C? 35°C? Which one is optimum?
11 November 2017 5,831 16 View
Is it necessary to lower the pH of Mueller-Hinton agar supplemented with 2% glucose in order to prevent bacterial contamination? Can we add conc HCL to lower the pH?
11 November 2017 3,936 20 View
Lets say 95% Ethanol or 95% Acetone?
09 September 2017 2,159 13 View
Generally, which solvent gives best results for a plant extract (larger zone of inhibition) against E. coli? Ethanol or Methanol?
09 September 2017 2,010 21 View
any disadvantages?
09 September 2017 3,241 20 View
Air drying in shade Air drying under sun Freeze-drying Oven drying at 60 °C Oven drying at 50 °C Please rank them.
08 August 2017 8,323 23 View
Agar pre-diffusion at room temperature or at 4°C?
08 August 2017 7,886 11 View
Which aseptic technique is best for agar well diffusion assay? pour-plate or spread plate? Please explain your answer. State references if possible.
08 August 2017 2,734 17 View
Which one disinfects faster and more powerful/effective?
08 August 2017 9,610 15 View
Select the answer out of A,B,C or D
08 August 2017 903 6 View
What is the best bacterial suspension of 0.5 McFarland turbidity (in microlitrers) to be inoculated to 25ml of molten agar in pour plate technique to achieve semi-confluent growth? Please post any...
07 July 2017 7,779 11 View
Would 1ml of 0.5 McFarland inoculum be too much for mixing with 25ml of MH agar in a 100x15mm petridish? I'm planing to use pour-plate technique for antimicrobial susceptibility testing with agar...
07 July 2017 4,537 13 View
Lets say 30g of plant material in how much water? to obtain 1000mg/ml?
07 July 2017 1,750 8 View
Any other recommendable methods? Is it alright just to leave the alcohol immersed plant extract to open air with the containers lid open for the evaporation?
02 February 2017 3,752 3 View
Do bioactive phytochemicals evaporate under room temperature in a non vacuum environment?
11 November 2016 5,546 3 View
Which is a better antibiotic to take as a control? Ciprofloxacin or Gentamicin in plant extract antimicrobial sensitivity testing against E.coli?
11 November 2016 9,142 3 View
How long will it take to evaporate 100ml of 95% ethanol in the refridgerator at 4℃?
11 November 2016 9,352 2 View
is it 1000mg/1ml? please show your answer with reference.
10 October 2016 1,429 6 View
Does autoclaving changes the pore size of these membrane filters
10 October 2016 888 2 View
In order to prevent drying of the antimicrobial test solution in the agar wells during incubation
10 October 2016 7,454 13 View
In case of pour plate method for agar well diffusion assay..
10 October 2016 8,158 16 View
freezer temperatures at -20 & -80? or is it best to store at refrigeration temperatures at 0-4 °C does cold temperatures have any harmful effect on phytochemicals of a plant extract containing...
10 October 2016 8,212 9 View
Can Streptococcus pyogenes and viridans be grown & cultured on Brain Heart Infusion (BHI) Agar for antibiotic sensitivity testing?
09 September 2016 9,703 4 View
Mortar & pestle with liquid nitrogen treatment or Electric grinder and blender?
09 September 2016 3,221 15 View
Any temperature conditions? Can bacteria grow in saline solution?
09 September 2016 5,567 6 View
Ethanol Methanol Isopropyl alcohol Please state the best one & rank them
09 September 2016 5,568 16 View
Does UV-irradiation increase or reduce the level of phenolic compounds like flavonoids and alkaloids in a plant extract?
09 September 2016 4,140 6 View
Dose filtering a plant extract with 0.45 µm syringe filter reduce the concentration of phytochemicals? Dose UV irradiation changes the chemical structure of phytochemicals in a plant compound?
09 September 2016 6,771 3 View
If yes, then how long will it take?
09 September 2016 1,191 3 View
- Please state any reference values - How to calculate LDL cholesterol in albino wistar rats?
08 August 2016 450 7 View
What are all the other alternative solvents to dimethylsulphoxide (DMSO) that can be used for plant extraction? Give a list of them.
08 August 2016 1,024 5 View
1. Air dried, powdered and extracted with alcohol 2. Air dried, powdered and extracted with sterile distilled water 3. Pure juice extract 4. Supernatant of pure juice extract Please select the...
08 August 2016 5,513 10 View
How long can we sustain klebsiella pneumoniae, streptococcus pyogenes and viridans streptococci bacterial slants cultures in the refrigerator?
08 August 2016 1,886 8 View
is it more than 1 ml or less? if less than 1 ml then how can we calculate µg/ml?
08 August 2016 5,194 5 View
Why do glass petri dishes crack from the bottom when I hit them with the bunsen flame (for further sterilization)?
08 August 2016 1,388 8 View
How to mix them both and make a culture medium that supports the growth of streptococci species?
07 July 2016 9,730 2 View
I'm planning to subculture bacteria in blood agar plates and store them in blood agar broths Can this be done?
07 July 2016 5,276 10 View
please state the safest methods
07 July 2016 7,370 4 View
Room temperature or refrigeration?
07 July 2016 3,583 4 View
I want to make broth from nutrient agar. Is there any way to stop nutrient agar from solidifying? any anti-solidifying agent?
07 July 2016 9,629 6 View
My plant extract is a solution & how to do the sodium hydroxide test to check for flavonoids? Please state the amount of 10% NaOH, HCL and plant extract I should take for the test? Do I need...
07 July 2016 586 13 View
Refrigeration? Freezing or any other?
07 July 2016 1,164 3 View
Please state any formular
07 July 2016 2,150 2 View
When mentioning scientific names like Sedum caespitosum (Cav.) DC. can we just mention Sedum caespitosum without (Cav.) DC? to make the name short?
05 May 2016 7,599 7 View
Any agar medium that support the growth of protozoa.
02 February 2016 987 3 View
