Hello, better for A 0.5 McFarland standard prepare by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl2•2H2O), with 9.95 mL of 1% sulfuric acid (H2SO4).

For bacterial suspension use saline water and also prepare above mentioned 1% sulfuric acid with the use of same water which you use for saline solution.

Good luck!

Hello, better for A 0.5 McFarland standard prepare by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl2•2H2O), with 9.95 mL of 1% sulfuric acid (H2SO4).

For bacterial suspension use saline water and also prepare above mentioned 1% sulfuric acid with the use of same water which you use for saline solution.

Good luck!

If you want to measure the turbidity in media, I recommend you to set zero reading of spectrophotometer with 0.5 McFarland standard of bacterial suspension in your media i.e Müller-Hinton broth.

Hi,

You can use your culture media such as Nutrient broth, Müller-Hinton broth or any media.

Hi

I agree with Darbinyan answer.

Fysiologic solution (0,9%)- 9 g NaCl in 1 liter sterile water

You should use saline. Depending on what type of bacteria you want to test there could be some other choises - like for anarobic or fastidious bacteria.

See detailed info in standard methods for antibacterial testing: http://www.eucast.org/ast_of_bacteria/disk_diffusion_methodology/

Hi,

I second the opinion of Jane. You will have to do some bg study (specific to your bacteria) and choose the right media.

better to use salin for this

For me, Muller Hilton broth or Nutrient broth will be a better media

Saline

You may use saline or Müller-Hinton broth then you decide which one better works for your experiments.

Saline

Saline will be more suitable for your AST.

I agree. You should use physiological saline.

I'd say it depends on what you're diluting your bacteria in. If you're making an AST where you dilute your bacteria in saline, use a McFarland made in saline. If you're doing some other experiment where you dilute your bacteroia in MH broth, go for a McFarland made in MH. It's all about controlling for the color and opacity of the medium you're looking at, just like you would make a baseline measurement on your spectrophotometer.

Muller hinton broth

It is a function of intended use but saline is preferred because Muller Hinton broth contributes to the turbidity to be measured and could result in false conclusions

the same result because liquide Muller Hinton or saline solution will be tared

Saline will be more suitable for your AST .

since you will be suspending bacteria after centrifugation in PBS or saline, PBS or saline should be used. Measuring absorbance values at OD: 600nm will provide readings. adjust to 0.08 to 0.1 for McFarlands 0.5