How to increase and reduce the pH of liquid agar medium?

More Mac Dz's questions See All

Which media is best for disk diffusion antibacterial susceptibility testing? Agar Bacteriological (Agar No. 1, Oxoid) or Müller-Hinton agar?

Which culture media is best for disk diffusion antibacterial susceptibility testing? Agar Bacteriological (Agar No. 1, Oxoid) or Müller-Hinton agar?

02 March 2020 4,461 3 View

Do the agar plates with inoculated disks has to be kept inverted during pre-diffusion in the refrigerator at 4°C?

Pre-diffusion of agar plates seeded with bacteria and inoculated disks for disk diffusion assay.

01 February 2020 711 12 View

Is there a difference between the quality of Oxoid™ Mueller Hinton Agar and other Mueller Hinton Agar brands/manufactures?

Has anyone observed a quality variation during antibacterial susceptibility testing like in disk diffusion assay? Oxoid™ vs Other brands?

01 February 2020 5,537 3 View

Which is a better disinfectant pure acetone or 70% isopropyl alcohol?

Which one kills bacteria & fungi faster & more effectively?

01 February 2020 1,493 5 View

Which type of petri dishes are more suitable for growing bacteria & for antimicrobial susceptibility testing? TC treated or non-treated?

TC = Tissue-Culture treated

01 February 2020 3,385 6 View

How to prepare a 50% DMSO plant extract?

How to prepare 50% DMSO from 100% DMSO? What is the solution should I use to dilute it? I have water and chloroform. The plant material is initially extracted in chloroform. Need to...

11 December 2019 9,831 8 View

How long should be a dried plant material extracted in chloroform & hexane be stable?

How long should it be good to use for antimicrobial activity testing? If the extracts were kept at room temperature (28°C ) & in the refrigerator 4°C?

11 December 2019 9,983 10 View

How to make 100 ml of Müller-Hinton agar?

Can I make 100 ml of Müller-Hinton agar from 38 g mixed in 1 L Müller-Hinton agar solution?

09 October 2019 6,189 7 View

Which is a better medium for preparing 0.5 McFarland turbidity standard for antibacterial activity assay? saline or Müller-Hinton broth?

07 August 2019 6,173 21 View

What would be the concentration of a 10μl aliquot taken from a 10 ml solution with a concentration of 1000mg/ml?

What would be the concentration of a 10μl aliquot taken from a 10ml solution with a concentration of 1000mg/ml?

07 August 2019 3,836 6 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Li Xi'an

Hi, if you want to increase the PH,you can titrate NaOH， on the contrary， you can titrate Hcl.

Pratik Roy

Add NaOH to increase pH and add HCL to decrease pH.

Sayan Bhattacharyya

add alkali and acid respectively...

Bhumi Bhatt

For pH any acid or alkaline solution you can use, Normally in practices HCl and NaOH are use for acidic and alkaline pH respectively. It is important that you set pH before adding agar powder in your medium.