Which aseptic technique is best for agar well diffusion assay? pour-plate or spread plate?
Please explain your answer.
State references if possible.
Spread plate is best as it is quicker and gives consistent results (so long as the inoculum is standardised) and you can immediately see any contamination.
take a look
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC378201/http://bitesizebio.com/21171/to-pour-or-to-spread-this-is-the-question/
Pour plating is usually used when you want to determine CFUs/ml (quantify a bacterial stock for example) or PFUs/ml (quantify a phage stock). Once it's pour plated, you can't really dig the bacteria out of the agar.
Spread plating is used when you want to isolate specific clonal colonies, for example, after cloning to select for cells that uptook a plasmid.
For Antimicrobial testing spread plate method used because we can check particular microorganisms sensitivity towards the antibiotics
it is to link
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC378201/
http://bitesizebio.com/21171/to-pour-or-to-spread-this-is-the-question/
excuse me
Dear Mac Dz,
Antimicrobial sensitivity we use spread plate because we need sensitivity for particular bacteria.. but for pour plate we goes for lawn culture....it is describe as appearance of bacterial colonies when all the individual colonies on a petri-dish agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering.
Spread plate is best as it is quicker and gives consistent results (so long as the inoculum is standardised) and you can immediately see any contamination.
Dear Mac Dz. Spread plate technique is better for antimicrobial sensitivity test.
Spread plate is better than pour plate (using a standardized inoculum) as it is quick, easier to perform and better to detect contamination
Spread plate technique is better and easier in terms of complexity. Pour plate is more laborious and requires skill. Moreover, contaminants are easily spotted with spread plate.
Since you need to check the activity by measuring inhibition zones I recommend you to use spread plate technique. Otherwise, in terms of observing and measuring inhibition zones, you may face troubles. And also one important point is checking contamination. It will be easier for you to observe and identify, whether there are any contaminations in spread technique.
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