Agar pre-diffusion technique at room temperature is  more effective than at 4oC because, diffusion increases with increase in temperature

Dear Mac Dz, Agar pre-diffusion technique at room temperature is better.

 The attached file may help you

Dear Mac Dz.. using agar pre diffusion method at room temp better.

Dear Mac, you can add 1% of Tween 20 to the culture medium, after inoculation, keep the plate at 4 oC for 4 hrs to allow pre-diffusion of the antimicrobial agent. Thereafter, put the plates in the incubator.

Haytham answer is ok. Tween acts like a detergent and emulsifiant and the temperature of 4 oC stops the bacterial growth until the antimicrobial agent diffuses in the agar.

Dear Mehlika,

usually after disk deposition all protocols define an incubation at 4°C for at least 2 h. I always had good results in particular assaying hydrophilic molecules against bacteria.

However, if your target are fungi, the pre-incubation at 4°C could be useless.

Bye

Federico

room temp

4°C would be better, for two hours followed by an incubation at the ideal temperature for the target germ. Good luck!

usually after disk deposition, keep the plate at 4°C for 2-3hrs to stop the bacterial growth and to allow pre-diffusion of the antimicrobial agent, . Thereafter, put the plates in the incubator at ideal temperature for the target germ. I always had good results.

Good luck

At 4° is better for 2-4 h

Drifa Yalaoui-Guellal

Hadj Ahmed Belaouni

Federico Baruzzi

Moad Farrai

Dear all

Thanks for the responses. Also should I keep the plates inverted during pre-diffusion in the refrigerator at 4°C?