Would 1ml of 0.5 McFarland inoculum be too much for mixing with 25ml of MH agar in a 100x15mm petridish?
I'm planing to use pour-plate technique for antimicrobial susceptibility testing with agar well diffusion method.
This will give you a population of ~10ˆ7, which is quite high for susceptibility tests. Check CLSI Methods (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically M07-A8) if you want to do this test properly. If you're working with anaerobes, check their method M11-A8.
This will give you a population of ~10ˆ7, which is quite high for susceptibility tests. Check CLSI Methods (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically M07-A8) if you want to do this test properly. If you're working with anaerobes, check their method M11-A8.
Dear Mac, as Fernando suggestion you should follow CLSI or EUCAST instructions for antimicrobial testing. For homogeneous distribution of bacteria on the surface of plates, we use sterile swaps. The bacteria prepared in McFarland 0.5 concentration inoculated by swap on the surface of mueller hinton agar plates.
Best regards.
Is going to be too high best option is to follow the CLSI guidelines.
It is too high. You can try with 0.1 mL spread on the surface of agar.
usage of swab is better than pour plate method. 0.1 ml many not be evenly distributed. I think so.
Please follow guidelines as recommended above. CLSI or EUCAST.
For EUCAST, follow the link below for guidance:
http://www.eucast.org/ast_of_bacteria/
Good luck.
http://www.eucast.org/ast_of_bacteria/
During my research work, I observed pour plate techniques show submerged colonies. It is preferable to use swabbing or spreading uniformly and 0. 1 ml should work.
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