I want to make broth from nutrient agar. Is there any way to stop nutrient agar from solidifying? any anti-solidifying agent?
Dear fellow scientist
If you want to make a Nutrient Broth...you need to buy a Nutrient formulation without the agar in it. The agar is the solidifying agent and no matter what you do, the Nutrient agar will solidify once the temperature reaches 40oC. Unless you hold it above this temperature, it will solidify. So the best thing to do is to get one without the agar. The composition is the same and the only thing that would be missing there is the agar
Dear fellow scientist
If you want to make a Nutrient Broth...you need to buy a Nutrient formulation without the agar in it. The agar is the solidifying agent and no matter what you do, the Nutrient agar will solidify once the temperature reaches 40oC. Unless you hold it above this temperature, it will solidify. So the best thing to do is to get one without the agar. The composition is the same and the only thing that would be missing there is the agar
Weigh the required amount in cold water as per needed volume. Then separate the supernatant clear liquid,, that is Nutrient broth.
If you want to keep molten nutrient agar in liquid state, keep it at 50 deg C in a water bath.
Is very difficult to separate agar and nutrients in media. I agree with Becky. Nutrient broth is evaluable in comercial formulation. Is very good media, we use it for several. xperiment.
Good luck!!!
If you want to separate the agar, prepare the normal concentration of nutrient agar in water (not boil), then filter the solution in filter paper. You will get nutrient broth.
Dear Friend,
If you want to avoid solidification physically and not to use then recommendation are temperature above 40. However I feel that you want to use the media as you have no broth available thus my recommendations are that there is no substitutions for that.
Better individually you can prepare broth adding separate components.
Also above method can be implied theoretically as agar is solid and would dissolve only on heating thus can be filtered, but robustness is a question along with the concentration variation in components while filtration.
Wish u Luck!
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