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Questions related from Ellie Major
Out of all that is approved and unapproved and also out of all that is proven and simply rumored to or that may work, what is a list of effective hair loss prevention agents? Preferably for male...
05 May 2016 900 2 View
I asked if isopropyl alchohol could be used in place of ethanol in an earlier posted question and it seems that neither is suitable to dissolve sucralfate. I will be making a solution that is to...
03 March 2016 4,692 5 View
How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?
03 March 2016 9,707 2 View
I have two problems given in my textbook that the book unfortunately does not give a good amount of information on. I have the answers in the back of the book, but I need to know how to do this....
09 September 2015 8,035 1 View
What are some useful books on the subject of Chemistry? Any recommendations are appreciated! What would be a good book (or books) for a complete beginner to learn Chemistry?
08 August 2015 8,845 0 View
Want people who have been through med school to list all the books they read while attending and the books that helped the most. Thanks!
07 July 2015 5,535 0 View
I would like to know how a childs father can be determined through a paternity tests. What is the full process? What are the names of the tests involved? What is needed? Is it always accurate? Is...
07 July 2015 1,232 0 View
I need some help determining the amount of each ingredient that will go into my solution. Here is the soluion criteria Component Amount (wt%) Volume Ratio ...
05 May 2015 8,630 0 View
If I have a powdered or ground plant, how can I make a petroleum ether extract of it?
04 April 2015 6,828 15 View
What would be the best way (or how you would personally go about doing this) to make a liquid solution using a powdered plant extract without subtracting from the plants natural medicinal effects...
02 February 2015 5,639 19 View
I have a project where I will be needing to dissolve a plant powder extract using dmso and I have a few questions. I am making a solution to apply topically so I would like to know: 1.) How much...
02 February 2015 8,938 11 View
I have a problem where I will need to convert micromoles per microliter into milligrams per milliliter. I am trying to scale up a solution where the original is 1μmol of Drug X in 5 μl ethanol. If...
02 February 2015 1,039 10 View
Hello, I believe my calculations are correct, but I want to be sure before I proceed. I will just take two random liquids and ask my question as thoroughly as possible. So lets take olive oil and...
02 February 2015 3,378 7 View
Besides cyclosporine, does anyone know of a substance that inhibits calcineurin? I am looking for anything that can inhibit it such as plants/herbs, oils, extracts, drugs etc. Please let me know....
02 February 2015 736 6 View
I am hoping to find somebody who might know the gossypol content of cottonseed oil. Is gossypol available in high quantities in cottonseed oil? If not, where might I find an abundance of gossypol?
02 February 2015 4,544 6 View
Hello everyone, This question is directed towards people located in South America. The study I will link you to below describes how capybara oil has been used traditionally as medicine in South...
01 January 2015 7,290 2 View
If I were to conduct an experiment where I would apply Aspartame (mixed with water) to the skin, and follow with electroporation, what do you think the likelyhood is that angiogenesis will be...
01 January 2015 8,027 3 View
I will be conducting an in vivo test to define transdermal delivery via topical/skin electroporation. I will be applying a drug to the patients skin and using an in vivo electrode to carry the...
11 November 2014 6,719 9 View
I would like to deliver the pdgf and vegf from prp into the dermis of human scalps and would like to know if you think it is possible to do so. Do you think that pdgf from prp can successfully be...
11 November 2014 3,728 1 View
I will be performing a test soon that I have been planning for awhile, though one are still hangs me up. The test will involve applying a solution to a human patients scalp, and electroporating...
11 November 2014 2,723 5 View
I intend on using calcium chloride for activation of platelets in prp. What liquid should be used to make 10% solution of ccalcium
11 November 2014 263 1 View
I have recently bought a drug (prostaglandin F2a) and want to make sure it is authentic. Is there a lab that offers a verification service? A lab that I could send a sample in and they would give...
10 October 2014 8,587 1 View
Hello and thank you for any help you may be able to give. I have ordered a drug, prostaglandin F2a, and I would like to know that I have received the drug I ordered. What can I do to test and...
10 October 2014 6,447 10 View
I have a simple question about the relationship of prp and angiogenesis. Here is a link to a study that provides proof of activated prp's ability to induce angiogenesis:...
10 October 2014 9,245 8 View
I will be using ethanol, proplyne glycol and water as solvents for the drug bimatoprost. Should I use denatured or undenatured? Thank you very much!
09 September 2014 7,301 2 View
For the application of hair regrowth, could you mix bimatoprost with emu oil and expect it to stay viable? Why or why not? Please and thank you!
09 September 2014 4,124 0 View
I want to mix a certain chemical with an oil, however I do not know if it will be viable after it comes in contact with the oil. Can someone tell me how to determine a chemicals stability in the...
09 September 2014 3,089 3 View
Proplyene glycol, ethanol and water.
09 September 2014 7,671 0 View
Thank you for helping me. What I want to do, is use 10 ml's of ethanol with 10mg's of pge2, but I am not sure if these preparation instructions will contradict or lessen the efficacy of the final...
07 July 2014 5,042 1 View
Should only sterile water be used when a substance says it is water soluble? I will be using PGE2 and don't want to ruin it's efficacy.
05 May 2014 2,879 2 View
I have a few questions that may seem like beginner questions, but I would really like to know what I must consider when making a solution and keeping efficacy. I will be trying to make a topical...
05 May 2014 6,850 1 View
Is it possible to induce hypoxia of the scalp or selected regions of the scalp? If so, how? The purpose would be to help secrete leptin, which is a newly identified anagen inducer. Is there a...
05 May 2014 4,289 3 View
I know this is a rookie question, but I would like to know how drugs such as sleeping pills that you take orally make their way to the brain. There is a particular study right now about how...
04 April 2014 4,462 1 View
I have a digital scale that reads in the format 0.00 and in grams. I need to measure 4.5 milligrams. Would I still be able to use this scale? Shouldn't it read 0.45 g for 4.5 mg's? I'd settle for...
03 March 2014 7,982 8 View
I have a protocol and experiment that calls for using 4.5 mg of collagenase. My scale only reads as low as 10mg. Will I be ok using 10 mgs when it calls for 4.5? Please don't say to measure 10...
03 March 2014 4,199 2 View
I need to know how this is done. Most cannulaes are over a foot in length and won't fit in an autoclave. Is there another way to sterilize it? Povidone Iodine?
01 January 2014 4,362 3 View
This is kind of a simple question, but I need to know how collagenase is used when digesting adipose tissue (from the abdomen if it matters). I know the powder is dissolved in DMEM or a similar...
11 November 2013 1,628 0 View
I will need some assistance here. I need someone help me to put real numbers in place of the variables in the following protocol. You don't need to know anything about this particular method or...
11 November 2013 1,078 3 View
The following is hypothetical, but still very important. Kind of fun to think of actually. So scientists at Yale have apparently used preadipocytes isolated from a bald rat's adipose tissue to...
11 November 2013 3,389 1 View
I need someone to help me with each step assuming that I have 20ml's of adipose tissue. There's nothing wrong with this protocol, I'm just really bad at filling them in and want to get this...
11 November 2013 5,820 2 View
Looking for a supplier to custom order DMEM from. I would like it to be pre-conditioned with FBS, Penicillin-Streptomycin and Amphotericin. I will be digesting adipose tissue and will need it to...
11 November 2013 5,516 5 View
Would adipogenesis occur if you were to inject freshly isolated pre-adipocytes from human adipose tissue? There are a lot of studies that say it will but I just wanted to ask someone here in...
11 November 2013 6,395 3 View
I would like to know what the tools this doctor uses in his liposuction procedure are. The description says the syringe is a toomey, but what kind of cannulae is this?...
10 October 2013 8,047 0 View
I need to know how I can isolate preadipocytes (as in the study linked below) from adipose tissue. Let's say I have tissue from liposuction as in this video...
10 October 2013 6,721 2 View
I would like to know how collagenase is used to digest adipose tissue. I've only ever seen it sold in powder form. Does this powder have to be diluted or mixed with something? Or is the powder...
10 October 2013 9,361 5 View
Before a bone marrow aspiration, how would you sterilize the needle? Not that it's a used needle, but just to be safe!
10 October 2013 2,401 1 View
I am wondering if anybody knows of ebay-ish site for biotech products. I have a few of the stemcell column free easysep magnets I am not using and need to get rid of them. Anyone know where I...
08 August 2013 2,487 0 View
I need to know how to isolate the MNC's from bone marrow. I know how up to a certain point, and I am hoping you can take a look and fill in the blanks. So I have 50ml's of marrow and am isolating...
07 July 2013 2,896 1 View
My instructions say in one step to "aspirate the supernatant completely". My instructions are below, as well as my question. Here are the instructions: 1. Determine cell number. 2. Centrifuge...
07 July 2013 7,891 4 View
I am having difficulty understanding how I can replicate a study's success. Could you take a look at this old study (patent really)? They have done what I hope to, which is achieve a volume of...
06 June 2013 6,271 1 View
I read a study (link below) where they cultured cells and re-suspended them in a buffer before injection. I am curious what buffers are safe for this? PBS? aMEM? What do you think?
06 June 2013 7,406 17 View
I know how to separate pbmc's from blood, but I am just wondering what the most efficient way of doing so with 50ml's is? I have a rotor for my centrifuge that fits 15ml and 50ml tubes. So if I'm...
06 June 2013 9,689 1 View
I recently contacted Miltenyi and asked for an estimate on an average PGMC cell count from 50ml of bone marrow. They replied and said that in theory, there are usually 1x10e8 mononuclear cells in...
06 June 2013 1,186 5 View
I am using the cd133 microbead kit from miltenyibiotec. Here's the...
05 May 2013 837 2 View
I need to know how to isolate CD133 cells from bone marrow. I will be using the isolation microbead kit from miltenyi biotec. There are a few challenges I have that I cannot seem to get a...
05 May 2013 4,713 6 View
I am just a little unsure of the specifics here. If I need 1 ml of peripheral blood mononuclear cells, how many milliliters of peripheral blood would I need to centrifuge? Also, what would be an...
04 April 2013 4,127 2 View
I am wondering which stem cells are referred to when talking about stem cells derived from adipose tissue. Anyone know? I know cd34 and cd133 positive cells are among them, but that can't be all...
03 March 2013 3,509 1 View
I am desperate for help on this matter. Can anyone help me to isolate adipocyte precursor cells from adipose tissue?
03 March 2013 9,928 1 View
I am having a little difficulty with the basics of isolating cd133 cells from peripheral blood. My goal is to end up with 5 milliliters(or more) of cd133 cells. I am used to separating cells using...
03 March 2013 1,915 2 View
Is it possible to get stem cells from just centrifugation? Here's a site where a plastic surgeon claims that the middle layer and the pellet of centrifuged fat contains a great deal of stem cells...
03 March 2013 664 2 View
Can anyone tell me how to isolate preadipocytes from adipose tissue? It is impossible to find a study or method for this. If you know, please help. Model is human, and downstream application is...
03 March 2013 5,397 1 View
What human stem cell is most similar to embryonic stem cells(in terms of multipotency/ multipurpose)? Nothing that is derived from umbilical cord blood or bone marrow. Peripheral blood and adipose...
03 March 2013 1,036 4 View
I am currently a student looking to get into the field of biotechnology upon graduation and am currently interning at a privately owned biotechnology facility. At said facility, there is an...
01 January 2013 9,113 8 View
