I will be performing a test soon that I have been planning for awhile, though one are still hangs me up. The test will involve applying a solution to a human patients scalp, and electroporating the then wet scalp to enhance transdermal delivery. What type of electrodes should I use or would be best to use? The goal is to not use penetrating needles or any painful electrode that penetrates the skin. It should be applied topically to the skin and the pulse will begin. Can you recommend any electrodes for this purpose and link to them please? I will be using likely the btx ecm 600 or the bio rad gene pulser (or gene pulser 2) Or possibly my biorad micropulser.
Please help me out on this and point me to some appropriate electrodes. Thank youvery much.
Electroporation, or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of ligand. Electroporation is the application of controlled electrical pulses to living cells in order to permeabilize the cell membrane for the purposes of transfection or transformation. These pulses are delivered to a pair of electrodes by a pulse generator. The pulse induces a transmembrane potential which causes the reversible breakdown of the cellular membrane.Caliper Electrode, Genepaddles, Petri Dish Electrode, petridish tissue electrode,Platinum Electrode For Tissue Slices, Single Adaptor Cable for Wand Electrode and Tweezertrodes are most commonly used for in vivo eletroporation for human purpose.
The delivery of genes and drugs directly into living tissues has significant implications in gene therapy applications, cancer treatments, vaccine development and transgenic animal production.Caliper Electrode, Genepaddles, Petri Dish Electrode, Petri Dish Tissue Electrode, Platinum Electrode For Tissue Slices and Single Adaptor Cable for Wand Electrode are non invasive electrodes and can be sued for human purpose.
But as I was trying to communicate, none of those electrodes are suitable for human scalps! Any specific suggestions? What about this:
http://www.btxonline.com/2-needle-array/
I understand that that particular electrode is meant for the needles to go into the skin, but for delivering a drug to the scalp of a human do you think that these would be suitable. Could you use them just to touch the surface of the skin while it has the drug applied to the scalp? Would that work or would these have to be inserted into the skin? I judt cannot find a suitable electrode!
http://cdn2.bigcommerce.com/server1600/a606a/products/66/images/41/450167_450168__14223.1410567291.1280.1280.jpg?c=2
Bigger clearer picture.
Hi Ellie,
Just follow the links, I hope that you can surely get some info regarding your work.
cheers...
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0056673
http://onlinelibrary.wiley.com/doi/10.1002/elan.1140020303/abstract
http://books.google.co.in/books?id=wKHjtndRSDMC&pg=PA101&lpg=PA101&dq=electrodes+for+in+vivo+studies&source=bl&ots=idBFF6aDWc&sig=Llo-xP__TeR_wPMR7bo05Y62bKE&hl=en&sa=X&ei=h3xsVNXEOYmxafmJgvgN&ved=0CFAQ6AEwBg#v=onepage&q=electrodes%20for%20in%20vivo%20studies&f=false
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