What is the most effective substance to prevent hair loss?

Out of all that is approved and unapproved and also out of all that is proven and simply rumored to or that may work, what is a list of effective hair loss prevention agents? Preferably for male...

04 May 2016 823 2 View

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

02 March 2016 9,614 2 View

What should be used to dissolve sucralfate for use in a topical application?

I asked if isopropyl alchohol could be used in place of ethanol in an earlier posted question and it seems that neither is suitable to dissolve sucralfate. I will be making a solution that is to...

02 March 2016 4,514 5 View

Calculate the ph of 5.00g of HBr in 100 ml of aqueous solution?

I have two problems given in my textbook that the book unfortunately does not give a good amount of information on. I have the answers in the back of the book, but I need to know how to do this....

08 September 2015 7,773 1 View

What book(s) would you recommend for a someone interested in learning Chemistry?

07 August 2015 8,778 0 View

Can someone, preferably a geneticist, explain how a childs father can be determined through paternity testing?

I would like to know how a childs father can be determined through a paternity tests. What is the full process? What are the names of the tests involved? What is needed? Is it always accurate? Is...

06 July 2015 1,150 0 View

What books did you read in med school?

Want people who have been through med school to list all the books they read while attending and the books that helped the most. Thanks!

06 July 2015 5,468 0 View

Need help with solution quantities. Can someone help?

04 May 2015 8,562 0 View

How can I make a petroleum ether extract of a plant?

If I have a powdered or ground plant, how can I make a petroleum ether extract of it?

03 April 2015 6,711 15 View

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01 February 2015 942 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View