Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
5,156 views 3,164 posts
Questions related to Virus
Can I measure the amount of AAV capsid with Bradford assay?
28 July 2023 7,315 2 View
I am looking for an opportunity to review Cyber Security papers for any Journal, or conference. Any recommendation will help me.
25 July 2023 614 2 View
Actually, I have trouble in finding the short sequence of hepatitis delta ribozyme and hammerhead ribozyme which are used in RSV reverse genetics, I searched for so many articles but just find...
24 July 2023 3,786 1 View
Research Aim: Most data transmission is monitored by a firewall, which can also recognise and restrict undesirable traffic. The goal of this study is to highlight the potential features of a...
22 July 2023 6,919 4 View
Good day everyone, Please I am doing transfection/ transduction, I want to check the MOI of the virus and at the same time perform viral concentration, but i am stuck up with the steps. please can...
22 July 2023 942 2 View
I am currently trying to use a commercial "PEG Virus Precipitation Kit" to concentrate my baculovirus. After I add the PEG to my viral supernatant I will get a very large pellet. However this...
21 July 2023 9,071 0 View
We are trying to create in vitro models of Foreign Body Giant Cells with the THP-1 cell line. Literature seems to be very muddy, and most in vitro models are done with primary cells. Has anyone...
19 July 2023 6,343 2 View
I am looking for a cell lineage infected by herpes simplex 1 virus.
18 July 2023 1,267 3 View
Looking to perform TEM on tissues that have already been processed via RNAScope. Are there concerns with viral and/or cellular degradation from RNAScope reagents that may prevent TEM from...
18 July 2023 4,111 1 View
What is the role of useful microbes in recycling minerals and nutrients and role of useful microbes in recycling minerals and nutrients?
14 July 2023 5,102 0 View
Hi everyone! I work with NK-92 cell line (ATCC) and am trying to transduce them with a CAR. After several attempts of transduction, I’ve achieved a place where I feel hopeless. I’ve tried to alter...
14 July 2023 5,762 0 View
PeiR is a lytic enzyme which is a methanogen virus that infects Methanobrevibacter ruminantium M1. Do all protease have active sites, if so how do I find it with the sequence only.
13 July 2023 8,546 4 View
I would appreciate help here in avoiding re-inventing the wheel. Has anyone used AAV to transduce THP-1 in suspension culture? I do not want to do this in activated adherent (macrophage-like)...
13 July 2023 4,563 0 View
Hi dear colleagues, Thank you for reading my question. I'm struggling to compare my basal transcriptome microarray data (Clariom S, human, Affymetrix) versus some brain or neuronal database to...
13 July 2023 3,909 0 View
(GitHub or installer)
13 July 2023 9,969 0 View
Please send a reprint of this article Thank you, Diana Carrano MPH, PT, FACHE
12 July 2023 7,164 3 View
A Complete detail of all the interactions present between the ligand and DNA.
12 July 2023 3,178 3 View
As chlorides are very corrosive, I am searching for an alternative.
11 July 2023 4,501 3 View
Hello , I am having trouble in differentiating U937 cells. I tried using various incubation periods and different PMA dosages—10, 20, and 100 ng—but it seems like many of the cells are dying...
11 July 2023 2,391 4 View
Does anyone know the diameter (size) of mouse heart endothelial cell?
09 July 2023 1,477 1 View
I am trying to optimize the 1-step RT-ddPCR system using Invitrogen™ SuperScript™ III One-Step RT-PCR master mix Kit for absolute virus quantification of the virus. But in most of the articles,...
08 July 2023 6,096 2 View
Please guide me on how can I find a topic for my thesis and where to start work.
07 July 2023 5,895 4 View
With the increasing need for sustainable agriculture and climate change resilience, how can plant breeders effectively incorporate complex traits such as drought tolerance, disease resistance, and...
07 July 2023 1,594 3 View
what are the right conditions for phage preservation?
07 July 2023 6,695 2 View