Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I'm looking for online database depository (possible for collaboration too) of actigraphy activities performed on the children and young adolescents during daytime (in school, house or any...
24 November 2022 1,441 0 View
Hi I want to evalute EGA with Rstudio in qraph analysis in the middle of analysis. I tried to run bootnet order, but it takes more than 50 miniutes ! and the only thing in console is "Generating...
24 November 2022 3,172 1 View
I wonder about the minimal size of a DNA FISH probe, taking into account the possibility to amplifiy the signal (like HCR). Most of the literature on DNA FISH probes I found accounts for Kb/Mb...
23 November 2022 7,102 3 View
With the aid of relevant illustrations
22 November 2022 6,065 6 View
I'm using a protocol in which I digest a antibiotic resistance gene with KpnI and BamHI and it leaves overhangs on the same strand 3'-5'. Then I make two fragments A e B as showed in the picture...
22 November 2022 3,334 0 View
Hi All, Has anyone successfully used any of the following amplifiers to measure ~0.8 nA calcium currents in ventricular myocytes under perforated patch (single electrode)? Axopatch 200B...
22 November 2022 6,192 0 View
Hi, there Does anyone has experience with (MPBIO) FastDNA spin Kit for soil DNA extraction intrinsic contamination? Salter et al. 2014 test it, and it had several intrinsic microbial...
21 November 2022 7,139 0 View
I would like to recover viruses from ground water to do metagenomic sequencing. From my reading I found the researchers used cellulose nitrate filter membranes. In Egypt the available filter...
17 November 2022 9,803 3 View
The cells are kept at RT in a desiccator (vacuum).
15 November 2022 4,977 2 View
This is a PBMC culture, after 10hours I observed these small things other than cells moving and changing shape. But please help me identify what type of contamination it could be? The media also...
11 November 2022 860 2 View
In order to measure cytotoxic activity of T cells, we are trying to set up the DELFIA Cell Cytotoxicity assay. After many different attemps, we are still dealing with a high degree of spontaneous...
10 November 2022 4,272 0 View
Hello. I am just curious if there will be changes in function/behaviour if you optogenetically activate a particular region of the neuron (e.g. dendrite), compared to activating the whole neuron...
05 November 2022 6,217 2 View
Order(symmetry) and disorder(random) characteristics exist in every radiation and matter.They may be represented by symbol - O and symbol - D ,respectively. Every Action or Event are the...
04 November 2022 3,926 2 View
I am using Thermofisher 5'-RACE PCR kit. In troubleshoot guide, the reason for incomplete sequence could be due to non-specific binding of AAP in poly-C rich region interior to end of RNA....
31 October 2022 1,059 0 View
We just started a new experiment regarding miR204 in my lab, & we want to see how much it is being expressed in our cell cultures. I tried extracting RNA using a PureLink kit from Thermo...
28 October 2022 4,985 1 View
We are looking for MinION sequencing but we don't have experience for which QIAGEN Kit to use. It's for Acinetobacter baumannii
27 October 2022 5,065 1 View
Hello, I am looking to produce a set of two scfv fused to an IgG2a (to dimerize it) but I know that there can be aggregation problems, between the two scfv and also between the VH and VL of the...
20 October 2022 6,439 2 View
Trying to identify source for 3 questions on sleep: Over the past [7] days, have you: had trouble sleeping? woken up for reasons during the night? felt tired during the day? Any clues? Thank...
18 October 2022 7,816 3 View
Hello! Dear Research Team, can you help me regarding below question with difined conditions. Two new researchers, Prof. X and Prof. Y are joining same lab and have been given a 15 meter x 20...
10 October 2022 3,869 2 View
Hello. I am testing a model fit in AMOS. The data follow a normal distribution and meet the assumptions of: linearity, multicollinearity and homocestality. I have used maximum likelihood (ML). The...
21 September 2022 216 2 View
I have a Flu B virus that shows a positive result in plaque assay, but when infected in MDCK there is no CPE show!
04 September 2022 1,698 0 View
I have a set of frozen mammalian brain and lung samples which I need to screen for RNA viruses. I have seen some protocols e.g. mag max core kit (MagMAX™ CORE Nucleic Acid Purification Kit Catalog...
02 September 2022 2,585 1 View
I have read about the IUPAC Stability Constants Database, see also: - https://old.iupac.org/publications/scdb/index.html -...
02 September 2022 7,476 0 View
In India especially northern states i. e. Punjab, Haryana & Rajasthan cows are infected by the LSD disease. With this disease almost 5000 cows are died. In India most of the rural tenant...
28 August 2022 4,905 0 View