Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I want to make a CRISPR knock-in S2 cell line, but efficiency is too low. I have figured out different ways to achieve it as: S2 cells synchronization with cholechicine RNase H II use in...
14 August 2024 8,538 0 View
Suppose i have two compounds H2O and H2O2 or CO and CO2, i want to make crystal how can i make in material studio?
13 August 2024 3,770 1 View
I am trying to stain MRC5 cells with CTB-FITC to look at its membrane with confocal microscopy. I would appreciate if someone can share a protocol with me. In my first try I had a very weak and...
30 July 2024 1,984 0 View
Hello everyone, I am currently working on a project using Silvaco TCAD and I need to measure the etch rate of a specific material during the simulation process. Could someone please guide me on...
18 July 2024 2,592 0 View
Hello, I am PHD student and my thesis about The impact of lactobacilli on pathogenic bacteria that cause urinary tract infections in women ( in vivo). What is the procedure for treating them with...
04 July 2024 3,697 0 View
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01 July 2024 4,148 3 View
The title of our article, 'The Remarkable Behavioral Effects of Naltrexone in Old Rats: On Anxiety and Empathy', does not appear in Google Scholar. Only the journal name appears. Additionally,...
27 June 2024 8,776 0 View
I am working on the population genomics of a mosquito species and have around 300 whole genomes from one species sampled from several populations. Coverage is 30X per genome and genome size is...
27 June 2024 8,690 0 View
I'm a newbie to time-kill test, and tbh I've read every article about synergy between two or more drugs together but I'm still unable to grasp the nature and the standard protocol of a time-kill...
26 June 2024 7,450 0 View
C57 BL/6j reproduction age
25 June 2024 6,359 0 View
We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...
24 June 2024 4,323 0 View
To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?
21 June 2024 822 1 View
Hello everyone, I am first year PhD student, and I should work with Qupath for part of my project ( for cell detection and quantifying), I watched many tutorial videos, I asked from AI, I sent an...
21 June 2024 8,927 0 View
Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?
20 June 2024 6,896 3 View
I am evaluating the apoptosis of two types of cells, which are derived from primary mammalian cells (fibroblasts). When I use the Annexin V/PI method, the image I get is like this. It seems not...
18 June 2024 5,688 1 View
Porn to men is not a big deal. They honestly can’t see how watching a sexy film can be any sort of reflection on their love for their girlfriend. Most don’t understand why she takes offence...
17 June 2024 9,561 0 View
I want to implement a dynamic, priority-based leader election mechanism for the Raft consensus algorithm. Each Raft node's priority will be determined based on six performance metrics. The...
15 June 2024 6,521 0 View
Can the Chiralpak AD-H column separate the enantiomers of 2-(2-nitro-1-phenylethyl)cyclohexan-1-one from a Michael reaction? For separating the enantiomers of the aldolization product...
13 June 2024 3,592 2 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
13 June 2024 6,159 0 View
Hello everyone！ I would like to ask how to culture Helicobacter pylori. At present, I have cultured H. pylori on solid medium, but there is no proliferation after transfer to liquid. What are the...
13 June 2024 484 1 View
After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?
12 June 2024 5,514 1 View
I have a plasmid. This plasmid has 2 promoters, the first one is T7P to express araC protein. And the second promoter is pBAD to express lacZ protein. Both promoters are on the same plasmid and in...
11 June 2024 9,812 3 View
Washington and NATO
06 June 2024 4,613 3 View
current statistics and literature
05 June 2024 2,757 2 View