To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?
Yes you can
I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....
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I need an oil that is inert to Gallium
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I have been trying to prepare DES solution using Choline chloride and glycerol at 1:2 molar ratio at 80 ⸰C temperature. However, the Choline chloride is NOT fully getting dissolved in Glycerol. I...
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For ex we have the following data on 2 types of interventions carried out to control smoking in individuals. We have the following outcomes Outcome of intervention A on 5 individuals (1=...
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I have isolated temperate phages who are forming turbid plaques. I am facing difficulty while concentrating them
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How to Isolate Sennoside A and Sennoside B separately?
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In India, under Section 57 of the Copyright Act, moral rights or author's special rights are: right to claim authorship and right to restrain or right to claim damages for distortion or mutilation...
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In your perception, which Indian company/companies were successful in large cross border acquisitions from 2003-11. Large connotes to M&A size above 100mn$.
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
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Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
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I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
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Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
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Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
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Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...
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I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
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I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
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Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...
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