How to solve problem regarding invitrogen exosome isolation kit?How to successfully characterise exosomes by western blotting technique?

29 June 2018 4,485 3 View

Relative Risk = 0?

What if the Relative Risk value is zero? Does that indicate there is no association between the risk and the disease?

28 October 2016 2,825 5 View

Lack of expected association?

22 May 2016 3,797 7 View

Does miscibility in water differ for surfactant solutions (cationic, anionic and nonionic) ?

three solutions are of cationic, anionic and nonionic surfactant. are there differences in miscibility properties.

01 May 2016 1,931 5 View

Does anybody know about any archives (preferably online) on "Health in India" dating back before 1800s?

I want to compare transition of diseases and risk factors in India and the West. I can find innumberable literature about the west but hardly any on India

22 February 2016 2,615 3 View

Can anyone suggest a good CSE/CTH antibody for Western Blot?

I have used ABCAM and ThermoFisher and both do not work that well. Using it on Human samples, mainly trophoblast cells.

25 January 2016 1,590 2 View

What libraries would make Java easy to use for scientific computing?

So a friend and I will probably get into a project where we try to make mathematical and scientific libraries for Java, possibly in native code. So a few quick questions that come to mind...

12 March 2015 4,392 11 View

Are there any fluorescent materials for a violet/blue to red conversion?

I've been looking into fluorescence, and I see a lot of work on the phosphor that we normally talk about, in CFLS, tube lights, etc, but I don't see any optical-to-optical conversion fluorescence...

23 February 2015 6,261 7 View

How can I assign positive or negative charges on a specific atom in a molecule?

31 July 2014 8,219 10 View

Can anyone help me find out the MO composition in a metal complex?

How can I find out the % of metal character and % of ligand character in a particular MO of a metal complex?I have done a few NBO calculation but It only gives the bonding composition.

27 June 2014 4,622 12 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View