Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I have been having an issue with my plaque assay for a few weeks now, I am working with a soil sample I inoculate a certain amount of virus PFU/mL into the soil then use some recovery method to...
31 March 2024 5,739 1 View
Hello. Can anyone please ell me what theoretical background I can use to measure perceptions and practices of teachers in interculturality?
28 March 2024 450 2 View
Dear Researchers, I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells. My aim is to engage with fellow...
23 March 2024 4,407 0 View
Here in the Philippines, a lot of people claim to be statisticians; however, they don't have ample units in the subject area of statistics but claim to have experience treating data. What should...
20 March 2024 3,887 0 View
If possible, could you share a link to an article or electronic resource?
15 March 2024 3,069 0 View
I want to make a 500mM stock solution of oleic acid and linoleic acid in 100% ethanol, both of which have been supplied as liquids. Can somebody give the protocol as I'm very confused as to how...
12 March 2024 7,423 1 View
book - anatomy
03 March 2024 9,823 0 View
Could ficoll be used as a substitute for percoll in dencity gradients when performing microglial isolation? Right now, we are trying to isolate microglia for flow cytometry. We are going to use...
25 January 2024 1,498 0 View
Hello every hope you all will be fine.iam reading a paper and iam confuse about that point 5x10^5 dilution.iam giving the method below please seniors help me and clear my mind about...
24 January 2024 4,426 3 View
Hi all, I would like to ask about the stability of reconstituted DQ Ovalbumin from ThermoFisher. The guidelines suggest storing the reconstituted product in +4 Celsius protected from light. I...
24 January 2024 3,495 0 View
I am going to utilize the Image J software to count a mixture of cells at the same time. Does anyone know how I can count them using image J? I know how to count just one type of cell but do not...
23 January 2024 7,032 3 View
I am actually wondering what other safe tricks I could use to dissolved my plant extracts, having used distilled water and 100% DMSO to no avail? I have gone from 5mg/mL stock to 1mg/ML despite...
23 January 2024 4,946 6 View
For my IFA, I am using Alexa Fluor conjugated secondary antibodies. In my lab, other antibody, which is Cy3 conjugated has been kept at - 20 °C in aliquots. However, on the company data sheet, it...
23 January 2024 5,180 3 View
I transfected pcDNA and Eprotein in 293T cells, after 48 hours the supernatant was collected and exosomes were isolated, after lysing the exosomes , an IP was performed on the CD1d, and the final...
22 January 2024 5,078 1 View
Can we measure the concentration of filtrate before standart curve?
21 January 2024 7,205 2 View
I am doing ddPCR for HIV targets (env, gag/LTR and 5'pol) with infected PBMCs. I am using uninfected PBMCs, SUPT1 cell lines as a negative control. I suddenly started having signal in my negative...
20 January 2024 4,543 1 View
What is the right solution for separating zinc from electric arc furnace dust powder or using this powder for AM 3D printing?
19 January 2024 3,284 0 View
I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...
18 January 2024 7,760 6 View
I differentiated C2C12 cells in differentiation medium for four days and now intend to induce myotube atrophy by treating them with 10 μM DEX for 48 hours. However, I'm unsure whether to prepare...
18 January 2024 3,208 1 View
patients taking biology therapy to treat many autoimmune diseases, so many immunological disturbances occurred in their circulation.
16 January 2024 6,671 2 View
Here is my problem: The day before transfection, I seed the HEK293FT cells into 100mm dish(cell density 4*10^6 cells), the next day before I preform the transfection, cell confluency like 90%, and...
16 January 2024 6,387 4 View
What if the Paired T-Test results are significant, but Independent T Test results are not significant? (Done in the same sample) Ex: Paired T-Tests done in the Experimental groups are...
13 January 2024 3,248 4 View
Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...
11 January 2024 6,372 0 View
the accepted definition of a quantum particle is subject to Schrödinger dynamics as opposed to Newtonian dynamics. But we can find many interpretations of the quantum particle concept which we...
10 January 2024 8,684 6 View