Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I'm using 0.1 ug/ml of biotinylated antibody conjugated with the 40 nm streptavidin gold nanoparticles, 100 ug/ml as monoclonal antibody for the test line and 0.25 mg/ml of Biotinylated BSA. I...
26 June 2025 3,859 8 View
After Tamoxifen (ICI 47699) is dissolved in corn oil, if it is not used immediately in mouse experiments, what are the key points to pay attention to in terms of storage conditions (such as...
23 June 2025 7,475 1 View
I have a question about recombinant protein SPR experiments: After the recombinant protein was pre-enriched recently, the SPR test results were abnormal (such as weak signal/high non-specific...
21 June 2025 9,969 1 View
Hi everyone, I'm performing the glycolysis stress test using the Seahorse XFp on primary normal human bronchial epithelial cells (NHBEs), and I'm running into some issues. After incubating the...
17 June 2025 1,984 2 View
I want to generate a custom polyclonal antibody for a peptide and I wanted to ask for the best company/companies based on personal experience. I'm looking for a reliable rabbit polyclonal antibody...
16 June 2025 8,121 1 View
Most females seem to derive no particular satisfaction from their manipulations of the male. (Alfred Kinsey)
15 June 2025 362 0 View
During my research, I've come to conclusion that hemi and semi nested PCR are pretty much the same thing. But sometimes the definitions are different and I'd like to know if there is some sort of...
05 June 2025 5,796 3 View
How is a bacteria antibody-based kit created using the lateral flow method, and how should carboxyl latex beads be incorporated? What is the method and procedure?
28 May 2025 6,343 1 View
When constructing risk scoring models (such as PRS) for hereditary tumors such as breast cancer and ovarian cancer, we found significant heterogeneity between multimodal omics data (including...
23 May 2025 3,530 1 View
I am investigating how changes in thyroid hormone levels (such as those seen in hypothyroidism or hyperthyroidism) might influence cognitive processes such as memory, attention, or executive...
22 May 2025 5,685 2 View
Hello everyone, I’m working on a CRISPR-Cas9 knockout project in PANC-1 cells using a Doxycycline-inducible lentiviral system. Initially, I was successful in knocking out my target gene using the...
20 May 2025 1,442 6 View
Call for Submissions – Journal of Natural Built Social Environment Health (NBSEH) e-ISSN: 3085-461X
17 May 2025 8,594 2 View
dear all, i have a manuscript of an original article to be published. Smartphone addiction and attention focus......can anyone suggest me a journal which is indexed either in scopus or WoSc with...
08 May 2025 6,241 2 View
Hi, I have carried out a cell fractionation on two tumor cell lines, analyzing four conditions: whole , cytoplasm, nucleoplasm, and chromatin. Subsequently, we performed a qPCR (DNA), but the...
07 May 2025 5,423 1 View
I need to convert my ELISA results for Thyroid Stimulating Hormone (TSH) from pg/mL to μIU/ml.
02 May 2025 5,135 2 View
Good day I trust this e-mal finds you well. I am doing my studies in business administration and I have adopted the questions you used in this article “FACTORS INFLUENCING SALES PEOPLE’S...
25 April 2025 8,757 0 View
Hi everyone, I'm analyzing a recombinant protein (40 kDa) using Dynamic Light Scattering (DLS) and I'm getting a hydrodynamic diameter of ~30 nm in the number distribution, which is significantly...
22 April 2025 8,513 4 View
Few males achieve any real freedom in their sexual relations even with their wives. Few males realize how badly inhibited they are on these matters. (Alfred Kinsey)
29 March 2025 5,624 8 View
need to prepare input file
29 March 2025 9,632 0 View
I need to utilize computational chemistry to study the catalytic process of para-orthohydrogen conversion involving catalysts. I have already found a relevant article (DOI: 10.1002/ntls.10002)....
27 March 2025 1,525 0 View
I want to know how to improve writing skills
25 March 2025 1,428 1 View
I have developed a lateral flow assay using the same monoclonal antibody for both capture and detection. The assay consists of three lines: one control line and two test lines (T1 and T2). While I...
25 March 2025 3,461 1 View
"PROJECT-BASED LEARNING INTEGRATED WITH ARTIFICIAL INTELLIGENCE GENERATED CONTENT ENHANCING DESIGN THINKING TRAITS, The subject I chose to teach is UI Design, but it seems that UI design is more...
22 March 2025 2,893 4 View
I am conducting a project which involves introducing the L812M Mutation into GRIN2A through site directed mutagenesis. I have been struggling with the mutagensis PCR, as only very faint bands are...
20 March 2025 7,464 1 View