When using provean bioinformatic tool, what is meant by the amino acid variation?

12 June 2024 1 5K Report

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

Ananya Mazumder

PROVEAN analyses and thereby decides if a single or multiple amino acid substitution or insertion or deletion (Indels) have any impact on the functionality of the protein. So PROVEAN decides it based on a threshold score, -2.5.

If the value (provean score) due to a specific amino acid substitution or having Indels is greater than -2.5, it will be considered as 'neutral' implying no such significant alteration in the protein activity! But if the value is less than -2.5, it should be considered as 'deleterious' indicating a significant change in the function of the modified protein.

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