We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing steps leading up to flow, dead (floating) cells would be lost, making the cell population seem more viable than it actually is. So, we purchased a no-wash cell viability stain but since we still plan on collecting cells from culture via trypsinization and centrifugation, we're still worried that dead and dying cells will not be represented accurately.
What is the most accurate way to perform flow cytometry after a drug sensitivity experiment? Ideally we could add a stain directly into the cell culture well and then do flow, but then attached cells would not be represented.
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