Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hello guy, I need your help to solve my problem in experiment. I got sample as culture cell line. I have to detect whether virus infection or not in this cell culture sample (pharmaceutics...
18 August 2023 7,099 3 View
I am enquiring regarding my research project what the meaning of the "microT" in the microT threshold is in the DIANA-microT tool is, usedfor KEGG pathway analysis.
18 August 2023 1,208 0 View
In several older labs I've been in, if you wipe a wet paper towel or place a wet hand onto the black bench tops, the paper/hand turns yellow. Anybody know what chemical(s) this is? I'm not sure if...
17 August 2023 1,482 0 View
I am looking for research papers published after 2018 that compare the relevance of Google Scholar (using it directly or via Publish or Perish) to other scientific bibliographic databases (such as...
17 August 2023 9,655 0 View
Is Digital India successful in rural areas and how rural marketing can be more effective in India?
16 August 2023 1,598 0 View
Hi everyone. I have performed a qPCR of OCT4, BMP4, ABCG2, CK3 gene and GAPDH as a reference gene. After I run all my samples, I just realized that NTC for OCT4 and CK3 showing Cq value but not...
15 August 2023 787 1 View
Hello everyone, I have five thymine (T) nucleotides in my gRNA, which I use for the adenine base editor. Due to PAM restrictions, I cannot use a different gRNA. I want to use AAV as a delivery...
15 August 2023 755 2 View
I am currently establishing a transgenic cell line (H9) using a plasmid containing Zeocin resistant gene. An antibiotic kill curve was performed ranging from 0.5 ug/mL to 1000 ug/mL. H9 WT cells...
14 August 2023 5,074 1 View
Hello everyone and I hope I find someone who has done this. Has anyone transfected THP-1 cells in suspension culture? If so, I would love a protocol.
13 August 2023 7,274 0 View
Dear all, For a current research project on hallux valgus surgery, I would love to have an overview over the radiographic classifications (IMA and HVA) for hallux valgus severity over the time in...
13 August 2023 681 2 View
Hello, I am doing an experiment where I pretreat mouse splenocyte-derived CD4 and CD8 T cells with metformin for 3h before running the T cell persistence assay. I noticed a decrease in OCR after...
10 August 2023 5,945 0 View
...other than Settler Colonial Studies? My name is Kikila Perrin, a settler of mostly Germanic and Celtic lineage living on unceded lək̓ʷəŋən and W̱SÁNEĆ territory (Victoria area, BC) doing my...
10 August 2023 4,644 1 View
Hi, I am generating stable cell lines (HEK293A) with my gene of interest using the AAVS1 integration system. The integration site is present on Chromosome 13, which is triploid in HEK cells. I...
09 August 2023 6,492 6 View
There was a need to suppress three genes (tKD) at once in tumor cells. According to the literature, there are no problems with triple (tKO) or even quadruple (qKO) gene knockouts. However, we do...
08 August 2023 6,442 1 View
I have a basic nonlinear model ( 📷). Dependent variable: crown diameter (cw) and independent variable: trunk diameter (dbh). I had 200 plots and within each plot I measured the variables: crown...
07 August 2023 6,334 1 View
#SARSCoV2 is airborne - I am interested in finding the most recent / definitive research into its nature and protection from transmission. Thank you.
07 August 2023 3,840 1 View
Hey I would be happy to receive help and guidance on a research question about the nymphs and the final solution. Is the extermination of the Jews, the "Final Solution" part of the Nazi ideology?...
06 August 2023 1,112 3 View
Is it possible to compensate for the effect or lack of immunity after the eradication process?
05 August 2023 9,948 2 View
It was mentioned ,"The experimentation consisted of batch cultures in 2-L conical glass Erlenmeyer flasks were filled with 2 L saline water" but it is not possible to have culture volume at 2L in...
04 August 2023 9,275 2 View
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02 August 2023 931 2 View
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02 August 2023 1,797 1 View
I am injecting 1.0uL of viral vector into the locus coeruleus and looking for axon projections in the BLA and IL. When I do immuno for the control virus (2.1x10^12 concentration) it all looks...
02 August 2023 2,016 0 View
My daughter is 13 with HFA. In the past year, she has created a group of "friends" she talks to during the day (she changes her voice and talks to them, and they talk back, etc.). Often at dinner,...
01 August 2023 2,989 0 View
Dear all, I encountered the reduction of CAR gene percentage from D5 60-70% to D12 less than 10%. It is not always seen in all my CAR constructs. I use 2nd generation LV system, with VSVG, or BaEV
31 July 2023 505 3 View