Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hello there! I have full access to several metabolomic (metabolite concentrations) and transcriptomic databases (FC and pvalues). I would like to integrate all these info in one to obtain not...
10 January 2023 8,373 2 View
I am trying to infect EO771.lmb cells with lentivirus to express luciferase and Tdtomato. My cells are not expressing anything. I tried spinning the virus for 90 min with media+polybrene, but it...
09 January 2023 2,261 1 View
Recently we faced an issue where we transduced primary mouse T cells with CAR constructs + a fluorescent tag. We tracked the expression of the tag on day 0, 2, and 4 post-transduction and the tag...
08 January 2023 229 0 View
Recently , clinical microbiology and immunology techniques used were mostly automated, and the conventional methods are almost left behind . Do think these new technology produces a well-trained...
05 January 2023 1,483 7 View
You are carrying out virus infections. Your stock contains 5 x1010 pfu/ml. What volume of this stock would you use to infect a tissue culture flask containing 2 x107 cells at an multiplicity of...
04 January 2023 7,274 2 View
Understanding the pathways and genes in HCC plays an important role in cancer prognosis there's some molecular genetic test for oncogene and gene expression
25 December 2022 2,804 2 View
The gifts (gold, frankincense, myrrh) were not of such a nature that they would've been of use to humble folks, except as items with a resale value to a broker. It has been suggested that the gold...
24 December 2022 9,060 18 View
current state of the art of the classical fragility contain epistemic uncertainty! That is because: - Displacement depend on the direction and magnitude of the force, so displacement based...
22 December 2022 6,702 1 View
We have a QIAcube HT and I can't seem to get consistent yield/quality out of it when isolating gDNA from CD4 T cells. We've moved to an off-board lysis in the deep-well 96 plate using buffer AL...
20 December 2022 2,566 0 View
Hi, I am just starting out with viral transduction and want to transduce primary human blood derived gamma delta T cells. I have read that gd-T and NK are harder to transduce than ab-T cells and...
20 December 2022 9,172 0 View
Due to the hazard classification of trypan blue we need to replace it with something less harmful and Erythrocin B seem to be a valid option but I find no detailed description on how to use it....
19 December 2022 7,516 2 View
Construction and Destruction words are complex and conjugate quantities such as life and death , begining and end etc. Birth of a child and formation- of Universe and Destruction of a child and...
15 December 2022 745 1 View
Hi, I produced a stable expressing Cas9 cell line. After transducing the cells, I checked the protein expression of my protein of interest and I understood that the transduced cells expressed a...
14 December 2022 474 3 View
I am preparing sample for SEM analysis, but lacking the facility of liquid.co2 and HMDS. So, is there any other method to dry the sample ? Your suggestions will be highly appreciated .
14 December 2022 6,600 6 View
I have been studying DLP/LCD 3D printing elastomers. I have tried using different UV curable materials such as HEA, EA, or polyurethane acrylate (PUA)-IBOA to fabricate UV-curable elastomers. The...
13 December 2022 6,584 2 View
After doing elisa with different dilutions of the sera (for eg, vacinee sera) against a target protein (for eg. sars cov2 spike), how do we calculate area under the curve?
10 December 2022 4,882 2 View
If there are two promoters inside, expressing the antibiotic-resistant gene and the gene of interest, does a polyA signal need to add after the first inside promoter? I appreciate any reply.
08 December 2022 2,680 0 View
I have an undergraduate who is interested in evaluating the effects of media components on protein expression. She is most interested in doing this in a baculovirus/Sf9 system and I am looking for...
05 December 2022 7,662 0 View
Urgent need for help! An article given the mean and standard deviation of Sleep latency and Sleep duration of PSQI, how can I convert the mean and standard deviation of the scores of these two parts?
01 December 2022 7,099 1 View
I required it for the in vitro lab experiment.
01 December 2022 4,925 3 View
Quantum and Quantization are the master kee of the Quantum Theory of Everything. Above anology may be understood by going through the attached papers as separate files.
28 November 2022 9,247 2 View
Quantum and Quantization are the master kee to explain the begining and end of whole existence of our Universe and Nature.
28 November 2022 8,282 4 View
I have prepared 1.2% avicel PH101 in distilled water, mixed with continuous stirring for 2 hours followed by shaking for 1 hour but unfortunately it is being sedimented instead of being dissolved....
28 November 2022 2,492 1 View
I used DnaSP software to perform a nucleotide diversity analysis on a viral alignment file. After observing the data I saw that some positions have high values (0.79) and some positions have lower...
26 November 2022 3,458 1 View