Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hi, I want to amplify the M13K07 helper phage . I used ss320 cells as a host. but I have not got a high titration. Does anyone has the experience to amplify the M13K07 ?
25 October 2024 6,580 2 View
When a patent is granted it is cited by subsequent patent publications as part of the 'state of the art' literature review. It doesn't seem that RG has access to the patent ref databases?
24 October 2024 8,366 3 View
One of the new students I am working with accidentally added jetprime buffer into a stock vial of DNA plasmid I am using to transfect for patch clamp. Will the jet prime buffer affect the plasmid...
20 October 2024 4,372 3 View
A healthy female, working in radiation field with a healthy,live issue reported to have tsh levels of 6.1 no other symptoms except occassional weight gain, hair fall and insomnia. No family...
19 October 2024 2,946 1 View
My PI wants to use expression plasmid for E. coli with two cloning sites. We have pColA-Duet-1 and pACYC-Duet, but in those are both of the sites inducible by IPTG (if I understood it correctly)....
14 October 2024 3,784 5 View
Dear Madam/ Sir, When I search for topics, where do I find the information about how many articles the site found? I only see the page number below, not the number of articles found. Also, when...
11 October 2024 7,189 0 View
I'm culturing THP-1 cells in a medium composed of 90% RPMI, 10% FBS, and 1% antibiotics (penicillin and streptomycin). When I checked my cells, I noticed some contamination (see picture), but I...
11 October 2024 6,850 0 View
Hello, I am looking someone who are working with Bruker MaXis series instrument. If there is someone I would like to ask if it possible to share with me a method file called:...
10 October 2024 9,036 0 View
I need to make a flavonoid-enriched extract from Plectranthus amboinicus through the partitioning technique using solvent extraction. I'll be using 80% methanol, petroleum ether, chloroform, and...
08 October 2024 9,946 0 View
I'm working on Mentha longifolia (horse mint) and trying to transform it using Agrobacterium via tissue culture. However, I'm having trouble growing it from seeds on MS media. The seeds either...
08 October 2024 4,437 1 View
need a journal on where a study was done on biscuits showing effects of moringa leave extract
07 October 2024 9,948 3 View
Take for example: A conference paper indexed in Scopus; can it be published into a Scopus indexed journal? Also, Material Today: Proceedings, is this a journal or considered conference proceedings?
07 October 2024 8,745 0 View
(lysis, wash, solubilization) in a single step because you know we lose conc. in each step I express it in E.coli, also i need to know that not harm my his-tag column
07 October 2024 9,602 0 View
I am working on pancreatic acinar cell function. I have checked so many papers it seems there is no acinar cell lines or organoids so far. I am wondering like-minded researchers who work on...
07 October 2024 8,940 3 View
I am encountering an error in FullProf stating "Some scattering coefficient not found" while attempting to assign Mo ions to specific ionic positions within the magnetic phase of a Sr2FeMoO6...
03 October 2024 8,354 2 View
As already discussed, the cerebellum is unlike the neocortex, for it is dedicated to motor control (Eccles 1967). To establish this control, circuits at the level of the Purkinje neurons are...
18 September 2024 2,238 0 View
Hi, In our lab, we are currently trying to express GCaMP (green fluorescent protein (GFP), calmodulin) in Lung Epithelial Cells. We plan to use either AAv or lentivector to do the expression. If...
16 September 2024 3,286 0 View
What are the critical limits for heavy metals in phosphate fertilizers? Concentrated superphosphate Phosphate rock NPK DAP
02 September 2024 6,502 3 View
I would like to inquire about the types of research that require ethical approval from the ethical approval committee. Specifically, I am interested in knowing whether cross-sectional,...
01 September 2024 2,643 3 View
I need assistance quantifying migrated PBMCs using a transwell assay. PBMCs (50 000 cells in 50 µl) were seeded in the upper chamber of a Corning HTS Transwell 96-well permeable support plate...
28 August 2024 5,810 1 View
The interaction between environmental and nutritional factors and reproductive success. And the latest advancements in reproductive technologies or interventions that can mitigate these effects to...
23 August 2024 8,511 4 View
I'm trying to do a retrograde tracing in developing mice, so it would be great to know the speed, and thus allow less transport time for very small pups and capture earlier ages. Thank you!
21 August 2024 5,389 0 View
Acceptance width for X and Y axis in ° Thank you in advance!
16 August 2024 9,321 0 View
Now the war enters the second year and no end is seen
14 August 2024 683 3 View