Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I am looking for a reference to support the statement that AAV vector transduction of neurons for ontogenetic applications is associated to a dose-dependent expression of opsins in the cell: i.e.,...
24 February 2022 3,427 0 View
Hi every one I would be very grateful for your expert help Am planning for in vitro experiment in which an ultrasound waves ( frequency ranged between 1_50 MHz and pressure amplitude ranged...
07 February 2022 7,862 5 View
I read an article that the higher RMSF indicates the larger perturbation of protein structure and hence indicating a better allosteric modulator. I wonder if the RMSF value is expected to be high...
30 January 2022 3,376 0 View
A known CKD patient (male) with OUP secondary to bladder Ca. He had bilateral moderate HUN. Left PCN tube insertion was successful. The right side ended up with iatrogenic duodenal injury and the...
10 January 2022 2,645 0 View
I did de novo assembly for sequences in Geneious. When I used minimum overlap identity of 99% one sequence was grouped in a different group compare to when I used 97%, all the other parameters...
06 January 2022 1,818 1 View
Hello fellow researchers, I would like to know if any of you have tried both a Qubit Fluorometer and also a Nanodrop on the same samples (DNA, RNA or protein) and which you deemed as more...
14 December 2021 7,841 4 View
I use VMD for analysis of NAMD molecular simulation. I have gotten a RMSF plot using a scrip via VMD. As it obvious, residue number 1399, attained maximum fluctuation in this plot. Now I wanna...
16 November 2021 7,800 3 View
I have an enquiry about using network-KDE standalone software (e.g., KDE+, SANET) for weighted crash data is there a way to use KDE+ to analyze crash data in terms of the severity index. Meaning...
10 November 2021 9,174 0 View
It's my first time working with this virus, I would like some advices!!
02 November 2021 2,944 0 View
can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks
30 October 2021 2,374 4 View
Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cels?? Thanks
30 October 2021 5,954 3 View
Hi all, We are embarking on a journey into the exploration of the respiratory phageome. To test our methods we are collecting representative bacteriophage. One has eluded us so far and that is...
19 October 2021 5,462 1 View
Hi, I would like to focus my dissertation on the phenomenon of the public voting 'NO' to the Colombian peace treaties of 2016. I have done some brainstorming: Causes: - Opposition campaign...
15 October 2021 6,366 4 View
Could You please give me a link to a source where I can get (for free) a data set of contrasted CT images of an abdominal aortic aneurism in DICOM (or which?) format manually segmented by a...
14 October 2021 9,102 3 View
I am trying to do calcium imaging from mouse adrenergic axon (NE). I injected “AAV5-hSynapsin1-FLEX-axon-GCaMP6s” to the LC nucleus in DBH Cre and TH Cre mice, unfortunately have not seen...
06 October 2021 2,320 4 View
My topic of my dissertation is empowerment of rural women with emphasise with produce herbal plants
03 October 2021 1,573 7 View
Hello all; Antibiotic administration in the drinking water of mice: How to improve drinking habits of mice? We tried antibiotic cocktail in the drinking water of SJL/J mice. Antibiotics...
01 October 2021 6,660 4 View
I tried to do amplicon sequencing for the RSV virus, I got only 22% coverage, kindly suggest the troubleshooting steps to be done. Sequencing steps in the nanopore was fine as I got complete...
23 September 2021 8,795 2 View
We are looking for some viral disease on Tuberose, what do you think of this symptom? The main goal is detection of Tuberose mild mosaic virus, please check this paper to compare symptoms: First...
23 September 2021 3,926 3 View
Hello everyone, I'm working on a project regarding the production of organoclays. What's your idea about filtration of sodium bentonite upon its purification, if we want to use a laboratory filter...
13 September 2021 7,097 0 View
Hi, I'm currently doing plaque assays for dengue and I'm not too sure on how I could calculate the limit of detection for the assay. For reference, I'm serially diluting (ten-fold) the virus six...
03 September 2021 8,396 0 View
My experiment design requiring use of virus components from start to obtain a lentivirus that carry designated DNA to create a stable cell line, before infection we usually use a 50c.c. 100kDa...
23 August 2021 8,614 1 View
I am studying for a master's degree in education in ASU, and I plan to start my research on the subject of adult continuous self-growth education. The focus of the study is on the appropriate...
23 August 2021 5,709 12 View
Hello, I recently came upon this phrase in the Addgene webpage describing lentiviral transduction. "Typically cells transduced with lower dilutions of the virus will have higher levels of...
20 August 2021 7,287 6 View