Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hi, I am trying to rescue VSV virus in BHK-21 cells using In Vitro Transcription system and co-transfection of N, P, L & G helper plasmids and FL VSV genome RNA's by Lipofectamine 3000....
24 May 2021 6,609 5 View
Hi every one. i am trying to write my thesis chapter about the important growth models influenced by public expenditure or government spending. Starting from Classical or Keynes, please guide...
19 May 2021 3,282 0 View
Hi I need the geometry with experimental data for validation, I really need it my pc had a virus and I lost all my work and in deadline to deliver it to school it will be a real help if anyone...
16 May 2021 4,437 1 View
There are observations that the places where TB is predominant , those country have almost 100% coverage of BCG vaccine for their children mean later stage all adult . Those country are...
15 May 2021 5,968 4 View
Why is neccesary or so important to have a virus titre of 100 TCID50/well when you want to perform a virus neutralization test? I have been trying to found an explanation in the literature but I...
29 April 2021 2,268 3 View
Hi all, I am struggling to amplify full length non-structural NS5B region (1.8kb) of HCV virus. I am using platinum super-fi taq polymerase to amplify this region. Also modify the cDNA synthesis...
21 April 2021 3,153 3 View
If we assume there is a mask contaminated with a specific virus and we spray that mask with a nanoparticle solution and this solution kills the virus / How was this done?
21 April 2021 8,380 3 View
Hello, I want to do the infectivity assay to test SIV virus stocks using TZM-bl cell and luciferase assay. Is there a direct way to have TCID50 directly form RLU? Once I'm gonna have the RLU how...
20 April 2021 1,505 1 View
Dear all, I am doing an RT-LAMP technique for RNA Tembusu virus detection. I used pre-adding HNB for the direct detection by the naked eye. In the beginning, the RT-LAMP reactions seem to work...
19 April 2021 9,435 3 View
Vaccines that employ attenuated virus may cause mild symptoms while those that employ adenovirus vector encoding the virus glycoprotein may cause more severe symptoms. Is it vaccine-related?...
17 April 2021 5,836 3 View
Seeking opinions on the role of humanitarian organizations on corona pandemic
16 April 2021 1,007 3 View
I need to increase the viral titer from 10^4 to 10^7 of the canine coronavirus-A72 cells model, how can I do this?
13 April 2021 2,192 4 View
I'm in need for protocols for the precipitation of phages (Without need for ultracentrifugation and alternatives for PEG)
12 April 2021 8,989 3 View
Normally, a virus –replicates or makes copies of itself and occasionally changes a little bit, which is natural for a virus. These changes are referred to “mutations”. A virus with new mutation is...
07 April 2021 6,696 29 View
Measles virus attaches with protein H to the surface of cell, once this happens it gives signal to the fusion protein F, which becomes active and initiates virus entry into the cell by membrane...
28 March 2021 7,932 2 View
The anti-Rotavirus Group A VP6 monoclonal antibody is the antibody against the group specific antigen of Group A Rotaviruses
26 March 2021 6,655 0 View
I am working on OBI among HIV patients naive to HAART. I expect to detect HBV DNA in HIV positive clients -ve for HBsAg but +ve for HBcAb using nPCR. My target regions are the BCP/PC, Core region...
21 March 2021 8,560 0 View
I am performing molecular docking for a peptide with a small molecule (ligand). After running the docking using AutoDock Vina, I visualize the docked position using LigPlot+ to identify hydrogen...
19 March 2021 6,511 3 View
I measured the DNA concentration using Nanodrop 2000 spectrophotometer and ROCHE High Pure PCR Template Preparation Kit. In the Nanodrop result, the screen shows contaminants in the DNA sample, as...
19 March 2021 9,229 4 View
we often use mengovirus or MNV .. to assess the quality of concentration and extraction of Norovirus given their similar physical and biological properties, please which virus is used to assess...
16 March 2021 658 2 View
Dear all members of the RG, My interest here is to have detailed information about the vacciine which is started to distribute in Ethiopia a few days ago. Of course, the ministry of health report...
16 March 2021 1,487 13 View
Hello everyone. My public groups have disappeared from Mendeley Desktop. Does anybody else have the same problem? Do you know how can I retrieve them?
15 March 2021 8,332 5 View
Dear All, I would like to check the cell viability of THP-1 derived macrophages after treatment with different stimulants. I am looking for a specific gene that is preferably upregulated in these...
11 March 2021 984 3 View
Iam writting related Thesis Research about New seurity threaths? Whethere its biological or nchemical weaponused for independent purpose. today its more transmitted, dangerous and active,. before...
02 March 2021 6,425 7 View