Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hi Fellows, I am trying to knock out a essiential gene. I have knock-in a stable mutant copy into cell by lentiviral infection, I would to subsequencially remove the endogenous copy by CRISPR-cas...
24 March 2023 361 0 View
I am trying to run a western blot to analyze rab4 differential expression after infection, but my final result is a lot of bands (just like a ladder) and I am not sure if that is unspecific...
24 March 2023 3,389 1 View
Sodium chloride often used for preparation of synthetic seawater. However, for complex preparation to mimic seawater (desalination) with some added foulants such as BSA, is there any specific...
19 March 2023 2,238 0 View
effect of an education program on urinary incontinence patients as systemic review research
19 March 2023 1,186 0 View
This is my first research topic and l need assistant with updated books and journals for literature review and how to critically appraisal the journals. Thank you in advance.
16 March 2023 9,280 0 View
Getting the highest band at 3.5kb but require the whole 5.1 kb operon for overexpression
15 March 2023 6,601 0 View
Putting it all together...
13 March 2023 3,789 4 View
Can anyone help me with an open acess journal with no publication charges/fees to publish an article on the impact of COVID-19 on pets and pet owners. The requirement of the journal should be 25...
12 March 2023 7,682 1 View
I recently noticed that the BSC-DREAM8b-Dust model can no longer be accessed. http://www.bsc.es/ESS/bsc-dust-daily-forecast Do you have any idea if it can be accessed from another database or if...
12 March 2023 2,602 3 View
I use biotin-hpdh 2 uM to biotinylate the protein and i block the membrane with BSA 3% 30 min and streptavidin-HRP 1:10000 , the result is black
11 March 2023 9,336 0 View
non
11 March 2023 8,916 0 View
tRNA derived fragments are a novel regulatory ncRNAs with distinct biological functions in cancers and stress-induced diseases. Our research group has worked on tRFs for a while. The major method...
10 March 2023 2,026 0 View
I guess I need the responses of more experienced peer reviewers who tried reviewing under WOS indexed journals. I cannot name the journal in particular.
09 March 2023 7,876 4 View
Hello, I am trying to find a mouse astrocyte cell line. I was planning to order C8-D1A from ATTC for some neuroinflammation experiments but the website was recently banned in my country (central...
09 March 2023 10,020 2 View
FMD is a contagious diease threat animal wealth please explain why control is difficult inspite vaccine manufactury developed?
07 March 2023 4,836 2 View
According to Protein Atlas in the attachments, ADAR1 protein is only located in the nucleus and ADAR2 protein has minor presence in cytoplasm. I'm wondering whether ADAR-dependent RNA editing is...
05 March 2023 9,616 0 View
Recently I keep getting citation listings for works I didnt write. I have been confused with Noel Cressie, who wrote Statistics for Spatial data. I did not write this book, although I previously...
28 February 2023 1,567 1 View
It might be strange in the secularized world to question the existence of God, but this question has confused and troubled people for thousands of years in the past. I wonder whether it is a...
25 February 2023 4,325 6 View
Anther cultures
25 February 2023 4,656 9 View
These symptoms is like mosaic virus
24 February 2023 5,613 3 View
Bacteriophage not proliferating after co-culture at 120rpm with its host in 20 ml Minimal M9 media in at MOI 0.2 what could be the possible reason
23 February 2023 4,388 1 View
Many people with functional diversity suffer from going to schools or institutes where they do not feel comfortable for various reasons. I would like to know what measures could be taken to...
21 February 2023 7,014 2 View
Which viral infection might be more severe?a transported virus from an animal to a human or a virus directly invade human?
20 February 2023 1,912 4 View
I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm...
20 February 2023 8,921 2 View