Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
So I have been performing stereotaxic surgery on VGAT-Cre mice. the virus I am injecting is a double floxed hm4d Gi - mCherry reporter. Whenever I finish infusing 250 nL of the virus into the...
17 August 2021 3,113 5 View
I have recorded data using both current clamp and voltage clamp. For Current Clamp, I found it very easy and straight forward to analyze action potentials and frequency in Spike 2; however, I...
17 August 2021 3,034 3 View
I'm doing research on Cacao swollen shoot virus. but right now I'm facing a problem that primers I use can't detect the virus. I have tried using several primers such as those described by Hoffman...
10 August 2021 7,304 3 View
Accurate karyotype information is essential for assembling a chromosomal level genome.
10 August 2021 4,884 0 View
I need this information to verify the recA deletion strain JW2669. PCR with the flanking primers gives a band at 1.3 kb which I suppose contains the cassette. Ideally, I'd like to know where the...
06 August 2021 551 1 View
I am working with the immortalised N/TERT1 keratinocyte adherent cell line and I need to perform a wound healing assay on them in the 96well black/clear bottom plate. Previously I was culturing...
05 August 2021 5,856 3 View
I am using an automation nucleic acid extraction system to extract HBV DNA from sera and used an externally added internal control along with it during extraction. In the automation nucleic acid...
04 August 2021 8,722 1 View
Hello, everyone. I am currently working on oncolytic herpesvirus 1. "To guarantee the genomic homogeneity and the purity of a virus stock, the initial inoculum has to derive from a single...
02 August 2021 282 4 View
I am working with U87MG (glioblastoma) cell line. I am using MEM with NEAA+ 1mM Na Pyr+ FBS as media. The cells grow fine in T-25 flasks. But when I seed them in a 96 or 12 well plate, the cells...
29 July 2021 8,884 2 View
I would like to know if it is problematic to find a cell pellet after having filtered, in a column, the solution of Candida albicans after having permeabilated the membrane via freezing / thawing...
26 July 2021 5,358 0 View
Hello, I am trying to titrate my P1 baculovirus. I followed the protocol provided. 1. I seeded subconfluent Sf9 cells in a 12-well plate and let them attach. 2. After removing the media, I...
22 July 2021 1,614 8 View
Hello, everyone. I am choosing a sonicator for lysing virus-infected Vero cells (to extract viruses) and resuspend virus pellets. As far as I know, there are two kinds of sonicators, probe...
21 July 2021 6,489 3 View
Hello I'm growing EBV-LCL cells but they are not growing well. Any idea why this is happening? Can it be caused by the reactivation of EPV virus stressing LCL cells? Should I use EPV antigen test?...
20 July 2021 4,354 3 View
I would like to study the effect of uremic toxin on atrophy of c2c12 myotube. I saw studies where treatment was done in serum free media. However it is well known serum deprivation alone cause...
20 July 2021 5,109 0 View
Without an available virus or pseudovirus, so no direct cytotoxicity assay. Generally I've seen that TNFa and IL-2 production is reported for CD4 cells and IFNg and TNFa for CD8 cells. I'm just...
18 July 2021 2,676 2 View
including, need of virus paasage in MDCK? dilutuion of serum 1:2 or 1:5 ? etc
12 July 2021 6,912 3 View
We would like to use pseudotyped virus to perform our experiments at the BSL-2 lab and I mainly find systems based on either VSV or HIV. Any suggestions on why choosing one versus the other? Thanks!
09 July 2021 7,442 3 View
Hi Everyone, I am planning to make organotypic slices from brain organoids derived from iPSCs to grow them on multi-array electrode plates. I always see in protocols the slicing of tissue in cold...
05 July 2021 8,337 0 View
If I take a frozen glycerol stock out of the freezer, can I leave to to defrost then immediately place it into the inoculating broth as my started culture and incubate it thereafter? I do not...
30 June 2021 5,654 3 View
I analysed some virus samples (d~190nm) in the DLS with a protein (d~7nm) as stabilisator. As the protein is present in such abundance the z-average is decreased significantly to
15 June 2021 9,324 3 View
We are using a replication-competent virus to express an immune-stimulatory protein on cell surface. We want to determine the functionality of the virus-encoded protein. Is it ok to fix the...
08 June 2021 3,007 1 View
I'm working on my master thesis in Fluid mechanics and turbomachinary field. My project is an axial water turbine called Kaplan turbine that besides having main runner has a sub-runner in series...
02 June 2021 4,992 0 View
To develop more resistance against corona virus by changing the food nutrient constituent or by biofortification or by genomic means etc
25 May 2021 9,987 4 View
While there are a lot of Avian Leukosis Virus (ALV) sequences in databases, there are fewer from Rous Sarcoma viruses (RSV). ALV is grouped within subgroups A to K except F, G, H and I. RSV is a...
24 May 2021 9,109 5 View