Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
hello everyone, I have a question about my experiment related to preadipocytes differentiation. I am using subcutaneous preadipocytes of cattle, when I passage them in 6 well plates and after...
28 April 2023 6,064 3 View
I have a problem in using U6 as a house keeping in determining miRNA levels in Caco-2 cells infected with rotavirus. There are different expression in the control (Caco-2 cells derivatives...
27 April 2023 4,611 0 View
What are the potential impacts of climate change on microbial communities and of microbes in improving environmental quality?
25 April 2023 3,395 1 View
My partner and I will conduct our undergraduate thesis on the efficacy of certain music genres in improving sleep quality. The results garnered from these would be used as a guide as we compose...
23 April 2023 1,042 5 View
Hello fellow researchers, I did a simple CUT&TAG for H3K27me3 in HEK293T cells. I checked my results using PCR. If you look at this picture that I attached, I first thought I had untagmented...
21 April 2023 6,151 2 View
I see lots of vacuoles kind of shape in the cytoplasm of my transfected cells, where I transfected it with a mutant protein. However, I don't see this pattern in Wild type transfection. What...
19 April 2023 3,328 3 View
I need manuscripts taught in North Africa, in Italy or European countries
18 April 2023 4,339 1 View
You shall bury him: burial, suicide and the development of Catholic law and theology Ranana Leigh Dine
14 April 2023 8,557 1 View
Hello, I have recently read about the reverse transcription activity of one of the subunits of telomerase. As far as I know, all reverse transcriptases so far are of viral origin, including the...
14 April 2023 5,866 2 View
If you are applying both wheat husk and pine needles to soil, can we collectively refer to them as "crop residues"? If not, what would be a more appropriate term?
13 April 2023 1,613 2 View
I am trying to extract Akata BAC from AGS-Bx1 cells and transformed into E. coli DH10B. EBV genes can be detected by qPCR with extacted product. But we cannot picked any EBV-positive DH10B...
12 April 2023 3,967 4 View
I am studing the funtion of an EBV encoding protein. M81-BAC was transfected into AGS, HK1 or HEK293t cells using lipofectamine 3000 and cultured with hygromycin B. GFP-positive cell colonies...
12 April 2023 1,797 5 View
I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?
11 April 2023 4,317 2 View
CRISPR KO Cells need to do single clone, then we can acquire 100% KO. How about siRNA? Can we do transfection to get a KD pool, then do single clone to have the 100% KO?
10 April 2023 1,825 1 View
What is the cause and effect of convection currents and what happens when convection currents are created in the air?
09 April 2023 8,046 5 View
Sometimes, your immune system can mistakenly recognize part of your body, as foreign. As a result, it releases proteins called autoantibodies to attack healthy cells. There are more than 80...
05 April 2023 4,679 3 View
how can I determine the bacterial count in food sample after applying bacteriophage asa biocontrol?
05 April 2023 9,866 2 View
What’s effect of tillage and crop residue management on soil physical properties & effect of tillage & residue management on enzyme activities in soils?
04 April 2023 246 3 View
What is the use of microorganisms to degrade the pollutants and role of microbial communities in the formation and decomposition of soil organic matter?
31 March 2023 7,951 3 View
I am attempting to express a protein using an Adenovirus vector. I have successfully inserted the gene of interest into the Adenovirus and used linear pAdeno to transfect the 293A cell line....
30 March 2023 3,750 0 View
What is the role of microbes in reducing environmental pollution & improving environmental quality & microbes play in the cycling of nutrients?
30 March 2023 2,491 2 View
For example, is always the absorbed dose in the bladder wall of 4 years-children upper than in 8 years-children? is there any exception in the absorbed dose in these organs with wall and content...
29 March 2023 1,520 1 View
What is most widely used precision farming system in agriculture & role of precision farming in Indian economy & what are the 5 R's of precision farming?
29 March 2023 4,317 3 View
my research work is on to check antiviral activity of plant extarct. can any one provide me demo video on antiviral assay protocol.
26 March 2023 8,269 0 View