Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Surface modification of silica nanoparticles with PEG?

I'm trying to coat my silica NP with diamine PEG, so i used GOPTS to add epoxy group on the surface of the NP then i'll add the PEG. I can not found protocol for SiNP coating PEG, most of the...

13 January 2021 4,915 4 View

Silica nanoparticles synthesis using Stober method with size rang 30-50 nm?

I'm preparing silica NP controlling the size by using different concentration of NH4OH. 1- 16.75 ml ethanol. 2-0.5 ml TEOS. 3- NH4OH (0.3- 0.5- 0.75- 1 ml) mixed for 24 hr then evaporate the...

27 November 2020 1,504 13 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

Problems in PCR using Folmer primers, can you help please ?

Hello, I'm using the classic Folmer LCO1490 and HCO2198 primers for a venerid bivalve ADN for two months but the results are still negative, I can't see the bands. 1. I tried to change the...

03 November 2020 9,190 3 View

How to culture prokaryote and eukaryote from the gut of marine organism?

Info regarding the type of media and culturing condition would be really appreciated. Thanks

21 July 2020 6,937 1 View

Calculating sample size for a multivariate regression in GPOWER?

Hello I currently have two dependent variables (with 4 measurements each) and two independent variables My question is how to calculate the sample size using Gpower for multivariate multiple...

21 November 2018 5,719 2 View

CDNA conc. for RT-PCR?

what is the recomended conc. to prepar cDNA to run rt-pcr for brain rat tissue.. i used 50ng/ul some primer gave ct value btw 20-22 but other above 30.. ?

30 October 2017 7,016 3 View

How do i increase the RNA 260/230 ratio ?

i did RNA extraction using differnt protocol triazol and invetrogen RNA mini kit .. the 260/280 ratio was 1.9 to 2.1 but for the 260/230 i got 1.5 to 1.8 in both protocols, should i consider it...

29 October 2017 4,780 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View