Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Can anyone help me with an open acess journal with no publication charges/fees to publish an article on the impact of COVID-19 on pets and pet owners. The requirement of the journal should be 25...
12 March 2023 7,595 1 View
According to Protein Atlas in the attachments, ADAR1 protein is only located in the nucleus and ADAR2 protein has minor presence in cytoplasm. I'm wondering whether ADAR-dependent RNA editing is...
05 March 2023 9,523 0 View
Recently I keep getting citation listings for works I didnt write. I have been confused with Noel Cressie, who wrote Statistics for Spatial data. I did not write this book, although I previously...
28 February 2023 1,476 1 View
It might be strange in the secularized world to question the existence of God, but this question has confused and troubled people for thousands of years in the past. I wonder whether it is a...
25 February 2023 4,250 6 View
These symptoms is like mosaic virus
24 February 2023 5,531 3 View
Which viral infection might be more severe?a transported virus from an animal to a human or a virus directly invade human?
20 February 2023 1,820 4 View
I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm...
20 February 2023 8,836 2 View
Recently, I was introduced to perivascular spaces (PVS) while studying lipopolysaccharide treated models with transmission electron microscopy (TEM). Erickson MA, Shulyatnikova T, Banks WA,...
17 February 2023 2,445 7 View
I did a plaque assay with an arbovirus in Vero cells. I infected my confluent cell monolayer with 200ul virus diluent followed by 1 hr infection with rocking to coat after each 15-minutes...
17 February 2023 8,218 0 View
I'm doing a systematic review of rehabilitation and return to sport after LCL injury. From the included studies I have, not all the studies have a rehabilitation-phased protocol. What is the best...
16 February 2023 8,377 8 View
Dear all, I have just purchased an aliquot of lncap from atcc, I thawed them and the result was disastrous. Briefly, these cells form big clusters and even when they adhere they maintain this...
15 February 2023 7,722 1 View
Hello guys I get a cryovial of HEK293 suspension cells which is low viability after thaw, like 37%. I culturing them in a t25 flask now, Will the viability getting high after cell revived? Is...
14 February 2023 1,575 9 View
Are there cytokines that are specifically expressed in humans and not other organisms?
14 February 2023 5,746 2 View
I am planning to do primary macrophage cell culture. I couldn't decide what to use to remove cells from the well plate prior to RNA isolation procedures, for example we usually use trypsin for...
07 February 2023 4,469 4 View
I'm trying to revive U937 cells, but I'm having a problem getting the cell line to develop normally. The growth media is RPMI-1640 from Sigma Aldrich with L-glutamine, 10% FBS added, as well as...
06 February 2023 1,493 2 View
I am now subculturing the Raji cell line (ATCC), however, I am having trouble since after splitting the cell line for subculturing, I saw that the cells were in the dead phase even though the cell...
03 February 2023 6,115 2 View
Hey, so I am writing a TCID50 protocol, and was curious as to why you want to add 200uL of total volume to the 96 well plates - is it just so the cells don't starve? I can't imagine another...
25 January 2023 162 0 View
I tried generating shRNA unique isoforms. I searched broad institute, and it gave 4 sequences. But when I did BLAST sequences from Broad institute it came up with 100% similarity for 2 isoforms,...
24 January 2023 9,119 0 View
Hey, I want to transduct K562 suspension cells with AAV. The vector is in serotype 9 AAV contains an apotosis-reporter and has a CAGs promoter. The titer of the AAV9 is 2.0 x 10^13 GC/mL. It...
24 January 2023 3,165 1 View
Mitochondria contain all the required machinery to synthesize ATP. They are also known as the "powerhouses" of the cell [Harper's Biochemistry]. With a healthy diet, containing adequate...
19 January 2023 9,647 0 View
Hello, I am trying to generate a mutant with the following retrovirus plasmid, but my cells are not resistant to G148. Can you please help me with any tips/suggestions? -pLNCX chick src...
18 January 2023 6,695 0 View
Jurkat T cell - MDA-MB-231 cancer cells
15 January 2023 6,224 2 View
Hi, I have three groups- one control and the other 2 disease groups. My main outcome variable has a correlation with age and duration of the disease and also there are gender differences. I ran...
13 January 2023 5,741 0 View
We performed mtt test for both two compounds soluable in medium and DMSO for cell culture applications. When we repeated mtt 2 weeks later, we did not see any effect compared to the control...
12 January 2023 3,388 1 View