Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Dear Experts, I would like to know is it possible to generate a cancer cell line (which is HLA-A:02 negative) to be HLA-A:02 positive by stably overexpressing HLA-A:02 gene into the cell line,...
22 August 2023 3,495 0 View
Hello guy, I need your help to solve my problem in experiment. I got sample as culture cell line. I have to detect whether virus infection or not in this cell culture sample (pharmaceutics...
18 August 2023 7,060 3 View
In several older labs I've been in, if you wipe a wet paper towel or place a wet hand onto the black bench tops, the paper/hand turns yellow. Anybody know what chemical(s) this is? I'm not sure if...
17 August 2023 1,456 0 View
Hi everyone. I have performed a qPCR of OCT4, BMP4, ABCG2, CK3 gene and GAPDH as a reference gene. After I run all my samples, I just realized that NTC for OCT4 and CK3 showing Cq value but not...
15 August 2023 754 1 View
Hello everyone, I have five thymine (T) nucleotides in my gRNA, which I use for the adenine base editor. Due to PAM restrictions, I cannot use a different gRNA. I want to use AAV as a delivery...
15 August 2023 721 2 View
I am currently establishing a transgenic cell line (H9) using a plasmid containing Zeocin resistant gene. An antibiotic kill curve was performed ranging from 0.5 ug/mL to 1000 ug/mL. H9 WT cells...
14 August 2023 5,005 1 View
Hello everyone and I hope I find someone who has done this. Has anyone transfected THP-1 cells in suspension culture? If so, I would love a protocol.
13 August 2023 7,233 0 View
Hello, I am doing an experiment where I pretreat mouse splenocyte-derived CD4 and CD8 T cells with metformin for 3h before running the T cell persistence assay. I noticed a decrease in OCR after...
10 August 2023 5,919 0 View
There was a need to suppress three genes (tKD) at once in tumor cells. According to the literature, there are no problems with triple (tKO) or even quadruple (qKO) gene knockouts. However, we do...
08 August 2023 6,406 1 View
I have a basic nonlinear model ( 📷). Dependent variable: crown diameter (cw) and independent variable: trunk diameter (dbh). I had 200 plots and within each plot I measured the variables: crown...
07 August 2023 6,299 1 View
#SARSCoV2 is airborne - I am interested in finding the most recent / definitive research into its nature and protection from transmission. Thank you.
07 August 2023 3,807 1 View
Hey I would be happy to receive help and guidance on a research question about the nymphs and the final solution. Is the extermination of the Jews, the "Final Solution" part of the Nazi ideology?...
06 August 2023 1,078 3 View
It was mentioned ,"The experimentation consisted of batch cultures in 2-L conical glass Erlenmeyer flasks were filled with 2 L saline water" but it is not possible to have culture volume at 2L in...
04 August 2023 9,239 2 View
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02 August 2023 892 2 View
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02 August 2023 1,752 1 View
Can I measure the amount of AAV capsid with Bradford assay?
28 July 2023 7,226 2 View
I am looking for an opportunity to review Cyber Security papers for any Journal, or conference. Any recommendation will help me.
25 July 2023 536 2 View
Research Aim: Most data transmission is monitored by a firewall, which can also recognise and restrict undesirable traffic. The goal of this study is to highlight the potential features of a...
22 July 2023 6,843 4 View
Good day everyone, Please I am doing transfection/ transduction, I want to check the MOI of the virus and at the same time perform viral concentration, but i am stuck up with the steps. please can...
22 July 2023 858 2 View
I am currently trying to use a commercial "PEG Virus Precipitation Kit" to concentrate my baculovirus. After I add the PEG to my viral supernatant I will get a very large pellet. However this...
21 July 2023 8,983 0 View
We are trying to create in vitro models of Foreign Body Giant Cells with the THP-1 cell line. Literature seems to be very muddy, and most in vitro models are done with primary cells. Has anyone...
19 July 2023 6,268 2 View
I am looking for a cell lineage infected by herpes simplex 1 virus.
18 July 2023 1,189 3 View
Looking to perform TEM on tissues that have already been processed via RNAScope. Are there concerns with viral and/or cellular degradation from RNAScope reagents that may prevent TEM from...
18 July 2023 4,039 1 View
What is the role of useful microbes in recycling minerals and nutrients and role of useful microbes in recycling minerals and nutrients?
14 July 2023 5,013 0 View