Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
01 March 2021 5,181 3 View
To assess the immunogenicity of the SARS-COV-2 RBD protein (CHO-derived) mice and Syrian hamsters were immunized two times (50 μg protein, mixed with aluminum hydroxide adjuvant ) on days 0...
22 February 2021 7,119 2 View
A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...
22 February 2021 3,242 6 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
18 February 2021 5,010 4 View
I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!
17 February 2021 8,963 1 View
Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...
13 February 2021 4,235 22 View
I was wondering if it was possible to put the samples through a short centrifugation, here is the protocol: 1: Add either “UTM” or “Water RNasi-free” to nasopharyngeal swabs. 2: Vortex. 3:...
07 February 2021 478 3 View
We want to extract RNA from virus samples, so we need to make everything RNase free. However, DEPC or RNase cleaning products are out of the question for us at the moment because of their cost. Is...
03 February 2021 2,499 4 View
Hello, I currently have my T4 virus in HEPES buffer. Unfortunately, there are always crystals that are contaminating my solution (due to the buffer in which the Viruses are) when I observe them...
02 February 2021 547 3 View
Hi I want to set up a virus neutralisation assay such as PRNT or FRNT at my lab (BSL2), but im uncertain of the bio safety requirements that has to be fulfilled in order to work with...
02 February 2021 5,755 3 View
Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...
30 January 2021 2,014 4 View
I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.
27 January 2021 8,875 2 View
I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...
26 January 2021 5,858 3 View
Vaccine manufacturer Merck Discontinues Development of SARS-CoV-2/COVID-19 Vaccine Candidates. The company found that the vaccine shots offered less protection than just contracting the virus...
26 January 2021 1,792 2 View
A usually overlooked region of a key SARS-CoV-2 protein is an important chink in the virus’s Armour. Neutralizing antibodies against SARS-CoV-2 block particles of the virus, which makes them some...
21 January 2021 8,176 5 View
I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...
18 January 2021 5,647 3 View
Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...
17 January 2021 7,078 11 View
Is a person who smokes at a higher risk of getting affected by corona virus than that a non-smoker?
15 January 2021 4,375 30 View
A candidate vaccine spurs both younger and older people to make antibodies against the coronavirus SARS-CoV-2, according to early trial results. The vaccine, which is under development by Johnson...
15 January 2021 8,305 6 View
I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...
14 January 2021 8,554 3 View
The new, more contagious variant of the virus that causes COVID-19 which initially detected in the U.K. and has now been spotted in many of the country. While some have referred to this as a new...
12 January 2021 8,957 5 View
I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?
10 January 2021 4,303 7 View
Any scale measuring stress, caution of exiting the home during COVID precautions, how cautious an individual is about COVID?
07 January 2021 2,510 3 View
I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...
06 January 2021 4,001 2 View