Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
Hello Guys, I am trying to isolate keratinocytes from skin biopsies for single cell sequencing. I would like to have just keratinocytes without any immune cells or fibroblasts. Can anyone suggest...
18 August 2022 7,322 2 View
I'm having trouble transforming my bacteria.
10 August 2022 2,321 0 View
Dear colleagues, I would like to know where/how I can obtain a MVA (modified vaccinia virus Ankara) vector and how to grow it. Thank you for your help!
14 July 2022 1,810 0 View
Dear All, Is it possible for a GPCR to localize in the nucleus in its inactive conformation, even though it is devoid of Nuclear Localisation Signal (NLS)?
04 July 2022 2,525 2 View
I have already acquired several ELISPOT plate images and I would like to count them again by my PC that is not connected to our ELISPOT reader.
23 June 2022 2,361 4 View
Hello, everyone! I am currently using the Large Klenow Fragment (lKF) for the detection of DNA and RNA oligonucleotides. I ran into a problem that can be explained with the 3'-5' exonuclease...
22 June 2022 8,034 0 View
Lineage tracing can be done by activating reporter gene which is positioned next to stop codon. Some people use cre-lox system as the reporter transcription controller, the others favor...
21 June 2022 8,443 0 View
Hello, all! I have calculated the percent of tranduced cells (%) and virus titer (TU/mL). I computed them after checking antibiotic selection process, which is a kind of functional titer, for 2...
17 June 2022 8,848 0 View
I have conducted foci forming assay to measure the titer of H1N1 on MDCK cells in a 24-well plate. However, there is no foci formation even in the virus dilution 10^-1 well, but the cells look...
15 June 2022 9,761 0 View
Determining impact of NP-40 and Freeze/Thaw cycles
09 June 2022 885 0 View
Is it ok to freeze cells again after less than one week of thawing them? and if not, what is the minimum time that should pass between thawing and refreezing again?
07 June 2022 5,653 1 View
specification needed: 1.solvent 2. dilution factor 3. acceleration voltage Thank you
24 May 2022 2,768 5 View
I am new to working with viruses, and my task is to infect lung cells with pseudo-virus. The procedure is as follows, 1) Culture HEK293T cells in 35mm dish, transfect with lentiviral spike...
21 May 2022 111 2 View
Hi everyone, I want to evaluate the proportion of ssDNA in my sample, is there a easy and accurate way to do that, thank you so much for all your help!
17 May 2022 9,334 3 View
Hi! I need help with virus quantification and I am confused with the units. In some studies virus's titres are written as 10E5 TCID50/mL in other studies 5 log10 TCID50/mL. Is it the same? Thank you
13 May 2022 7,962 2 View
Hi all I am doing ELISPOT for the isolated spleen and BM cells of mice to evaluate IgG and IgA Antibody secreting cells against pneumococcus. I immunized the mice earlier. and now at the day of...
10 May 2022 4,502 0 View
I am working on developing a POC (lateral flow immunoassay) which uses blood as sample. The problem I face is that when the Test is initiated, the Nitrocellulose pad is having the blood particles...
13 April 2022 8,677 1 View
Apple's M1 mac is there in the market since 2020, but its application and compatability with bioinformatics analysis tools is scarcely discussed. For example, is it possible to index a human...
05 April 2022 8,626 16 View
For a biological experiment I am trying to calculate at which angle I should set my rocking plate to create a specific flow in my microfluidic chip (gravity driven flow) I used The Bernoulli...
01 April 2022 2,599 1 View
Sea surface temperature, chlorophyll, winds, altimetry observations are done by NASA, ESA, JAXA, ISRO and a few other government institutes. Commercial satellite data providers provide very...
19 March 2022 8,842 2 View
Focusing on the vulnerabilities in consensus protocols
16 March 2022 5,125 1 View
I need to find out the occurrence of specific sequences across the genome in both strands. I'm able to retrieve the FASTA sequence of the +ve strand from the NCBI reference genome. How to get...
15 March 2022 8,286 4 View
Hello everyone, I am developping a TCID50 test for CMV virus, cytopathic effect should appear 5 days after inoculation. I wonder if it's possible to maintain MRC-5 cells at a concentration of...
15 March 2022 2,342 0 View
I am currently pursuing my research on the dengue-causing mosquito at its various stages. I'm studying the effect of microbial secondary metabolite-based nanoparticles against the vector that...
05 March 2022 7,168 2 View