Review and cite VIRUS protocol, troubleshooting and other methodology information | Contact experts in VIRUS to get answers
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Questions related to Virus
How does the interaction between plant pathogens and their host plants influence the development and spread of plant diseases, and what are the underlying mechanisms involved?
07 July 2023 2,163 1 View
I'm working on the expression of gH2AX protein in primary cells using the western blot. unfortunately, with normal ECL I couldn't detect any band! and with sensitive ECL I just see false...
03 July 2023 5,156 3 View
How can explain achieving the distance of 15 cm between the lines of wheat a maximum flag leaf area compared to the two distances20and 25 cm? Is it bkz the Less plant density inside the line 15cm?
01 July 2023 6,590 3 View
I am writing a review article, I want to know if it is common to use a general 3d structure of a virus in the publication. (of course by mentioning the reference)
30 June 2023 7,845 1 View
Dear colleges, Do you have experience using ExpiFectamine 293 Transfection Reagent for the trasnfection of Hec293F cells? I have a Expi293 F transfection kit...
29 June 2023 285 4 View
Give me answer in broadly
27 June 2023 2,365 1 View
I did the staining part. But I am facing the issues with the counting. I used the Hemocytometer for counting but I could not able to see the grids? Is there any other ways to count? I have used...
27 June 2023 8,720 0 View
The systems administrators have caused more attack to the system than the external hackers over the years. Researchers have equally updated literature on new approaches to attack the menace....
26 June 2023 8,708 2 View
Women empowerment is reflective or formative, which one?
23 June 2023 770 4 View
Hello, I have non-adherent cells that I want to do mtt assay and they are suspended in media, I need a special protocol.
21 June 2023 8,438 4 View
Hi, How long do you usually rest cryopreserved dendritic cells after thawing? And what cell culture media does it need for resting? Thanks!
19 June 2023 1,596 0 View
I know its specific location.
17 June 2023 6,847 7 View
I just send some samples for the SPR experiment using BiacoreT200. I received bad results with very high Kd in the positive analyte Ctrl and also non-specific binding occurred with other analytes...
14 June 2023 826 3 View
I work for an entity that provides rapid response for public medical emergencies. I'm looking to see if anyone has information on ways in which to improve response times for rapid gearing up of...
13 June 2023 8,296 1 View
I have a results from qpcr experiment for siRNA after transfection, how to know the effect of siRNA in Knock down from qpcr results
11 June 2023 9,606 2 View
I designed a primer pairs with the expected size of my product is ~750. I checked with primer-BLAST and it returns only 1 product. But when I do PCR and agarose gel electrophoresis, I see 3 bands...
09 June 2023 2,034 3 View
Are δs positive or negative for the spontaneous process and does entropy have to be negative to be spontaneous?
09 June 2023 8,247 1 View
I am uncertain about using HEK293 cells and MDCK cells for transfection using a lipofectamine reagent with a GFP tag plasmid. Both cell lines are mammalian cells. Please your response is appreciated.
07 June 2023 5,588 3 View
After performing some siRNA knockdown studies and overexpression studies I wanted to check my target protein localizing to the nucleus. After that I wanted to know the effect of localization on...
03 June 2023 1,105 0 View
I want to automate the measuring of neurite outgrowth in PC12 cells. Is there any programm or fiji plugin that can measure cellbody length ,neurite length and number of neurites in a unstained cells ?
02 June 2023 7,113 3 View
Dear all, I am working with exosome purification using Tangential Flow Filtration and concentrating the samples using 750KDa Hollow fibers modules. I don't want albumin to be present in my final...
01 June 2023 668 0 View
Hello, How would I determine the # of amino acid residues in a sequence? Is there an easier way to count them? I'm trying to calculate the molar extinction coefficient for AAV. The MW of the...
01 June 2023 1,863 4 View
Hi, I will do transfection with fugene but, I tried to enter three plasmids in cell. There is no selection part of plasmids, and I will try to plasmids enter to in cell one by one. How to do...
01 June 2023 1,894 3 View
Ecuador asumió la Dolarización para estabilizar su economía en el año 2000 cuales son resultados luego de 23 años del uso de esta política económica
01 June 2023 8,883 0 View