I'm working on Mentha longifolia (horse mint) and trying to transform it using Agrobacterium via tissue culture. However, I'm having trouble growing it from seeds on MS media. The seeds either don't grow well or they become contaminated. DDoes anyone have experience with horse mint and can provide the whole method for successful tissue culture?
Grow Mentha longifolia from seeds for tissue culture:
Sterilize seeds using 70% ethanol for 1 minute, followed by 2-5% sodium hypochlorite (with a few drops of Tween 20) for 10-15 minutes. Rinse 3-4 times with sterile distilled water. Place sterilized seeds on 1/2-strength MS media supplemented with 0.5-1% sucrose and 0.8% agar. Avoid growth regulators at this stage. Maintain at 24-26°C under 16-hour light/8-hour dark photoperiod. Ensure strict aseptic conditions to prevent contamination.
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