I'm trying to do a retrograde tracing in developing mice, so it would be great to know the speed, and thus allow less transport time for very small pups and capture earlier ages. Thank you!
I have a preprint on IS COVID AS LETHAL AS THE SPANISH FLU. I have uploaded the printed, substantially revised paper and wish to delete the preprint. Many thanks for your help....
24 June 2024 2,604 0 View
Hello everyone, I am relatively new to the field of fluorescence microscopy and subcellular localization analysis. Recently, I conducted experiments on HEK293 cells wherein I labeled both the...
24 March 2024 4,559 4 View
Dear community, We have analyzed several bacterial species using WGS and now wanted to determine both ST (MLST) and CT (cgMLST) using SeqSphere. The ST identification has generally worked...
15 October 2023 8,280 0 View
I'm working in an industrial field trying to improve some products and amongst them is Giemsa stain. The problem is, our solution cannot stain chromosomes of peripheral blood cells but control...
19 September 2023 9,258 3 View
Hello, unfortunately I have been facing problems with Mendeley since I started using it 1,5 years ago... Now I wanted to get back to it once again for a bigger lab report I have to write and I am...
19 March 2023 3,258 1 View
As I travel the world working in well established International schools as both an educator and administrator, I stumble on this issue without exception. The IB PYP philosophy often does not...
18 January 2023 6,542 0 View
In my current work I am using MMI MembraneSlides for LCM. The tissue is paraffin-fixed and I want to stain it with immunohistochemistry. Is it possible to treat the MembraneSlides with TE buffer...
16 June 2022 3,786 0 View
Hello, We are currently conducting a study to test how well bacteria and cells attach to different surfaces. We are also using a co-culture model with fibroblasts and S. gordonii. Does anyone...
10 October 2021 3,701 3 View
It was found on strawberry leaves (Fragaria annanasa).
30 September 2021 8,191 0 View
It has been identified on tomato leaves (Solanum lycopersicum).
29 September 2021 5,137 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View