Acceptance width for X and Y axis in °
Thank you in advance!
Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...
02 June 2024 5,489 2 View
My aim is to simulate cnt with aminoacids(histidine) in water solution. I`ve made two separate topology files(15 molecules of histidine with pdb2gmx command and cnt topology with x2top) then merge...
20 April 2024 1,760 0 View
I use BG 11+ Media but with an alternative trace metal mix.
17 March 2024 9,253 3 View
My protein is UFM1 tagged with Twin-Strep tag. I double-checked and my tag is not cleaved off or hidden. I made purification with IBA kit (Tactin XT 4Flow high capacity spin kit) with subsequent...
26 November 2023 4,087 3 View
Good day, I created a grid using the Autodock tools and then using those coordinates and size I created a configuration file for Vina. But in the results, the location of the ligand do not match...
12 November 2023 2,831 2 View
I am working in Paediatric Outpatient Physiotherapy department with children with Lower Limb problems. As a part of our help to children we are running 6 weeks strengthening groups. The question...
19 September 2023 2,570 0 View
I was wondering if the amount of sodium azide in primary antibodies for IHC can affect HRP activity in secondary antibodies, as I wash slides several times between the primary and the secondary...
12 September 2023 9,464 1 View
I am a medical doctor and I do not have any experience in IHC, only toluidine blue and HE staining. Can you please share a protocol with me? Thank you in advance
12 September 2023 7,596 2 View
Hi everyone. So I have performed MD simulations on desmond of my protein bound to a mutant peptide? My objective is to elaborate how the mutant peptide is inducing conformational changes in the...
21 August 2023 7,117 1 View
Hi! I ran MD Simulations of protein structure through SCHRODINGER software. The protein of interest has a lot of loops. I am getting very high RMSD of protein after simulations. How to solve this...
06 August 2023 7,344 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I will be doing an XRD on a sample which is powdered in natured and is a hydrogenated compound. I would like to know how we can understand the individual components and components by using the...
28 July 2024 4,548 2 View
