(lysis, wash, solubilization) in a single step because you know we lose conc. in each step
I express it in E.coli, also i need to know that not harm my his-tag column
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
thanks
25 June 2024 4,667 0 View
My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...
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Hello everyone! I want to learn python programming language for conducting exploratory analysis of the Skytrax reviews about airlines. Can anyone guide me which website or platform is the best for...
29 January 2024 4,455 5 View
I have paired end reads using target capture approach for 300 samples on 40,000 conserved regions/baits. I look at the baits and flanking for variable sites to reconstruct trees. The baits were...
22 January 2024 7,499 1 View
Here I have given the copy of the error which i have mentioned . Executing Formchk => "C:\G09W\formchk.exe" /3 "C:\Users\DELL\Downloads\chloroform_new_uv - Copy.chk"...
16 January 2024 888 0 View
Stander Curve utilize in ELISA Techniation
20 October 2023 3,286 2 View
Hi I'm looking for a scholar made a questionnaire on the effectiveness of EFL listening on listening anxiety and foreign language enjoyment. Can anybody help me with my PhD study?
02 August 2023 1,831 3 View
intra -IRV longs ring md^2
22 July 2023 3,198 0 View
I am doing a experimental study and multi-group analysis, is it possible that we can use different sample size in two different groups?
21 July 2023 628 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View