The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
1,613 views 3,440 posts
Questions related to Transfection
Dear colleagues, I am working on a project in which I want to deliver oligonucleotides (specifically, molecular beacons (MBs)) inside different protist species. To do so, I am going to use...
19 March 2024 2,748 0 View
What is the bubble in the cytoplasm as the figure I attached below? It also can be released into the culture medium after the cell dead.
19 March 2024 1,387 0 View
I need to submit some IP protein G beads for mass spec analysis. However, the mass spec has decided to be not available. Can I freeze the IP beads at -20C ready for the mass spec? If not, what is...
18 March 2024 7,596 0 View
Accurate way to determine the cleavage site of the protein if the cleavage can not be determined by Mass Spectroscopy?
16 March 2024 5,185 1 View
Hello everyone I am working on amplicons for species delimitation on corals. My supervisor want me to make it through a 4 primers PCR on 4 loci (CR, ITS, ORF and ATPSbeta) I have been trying...
02 March 2024 5,260 3 View
Considering the cleavage sequence for TEV ENLYFQ/G-S, if insteadt of G or S you have any other aminoacid, how is this affecting the cleavage?
01 March 2024 1,625 3 View
I am currently setting up a two step treatment on HepG2 cells in a 96 well plate, using an antioxidant and a drug. Majority of the literature that I have consulted mention a pretreatment with the...
27 February 2024 5,939 1 View
We are going to conduct a study on the antidiabetic activity of sea urchin extracts and it was suggested that we use cell lines to determine the activity of our extract. Are there any laboratories...
26 February 2024 8,725 1 View
I am in the process of learning how to culture MSCs that I have flushed from the bone marrow of mice femurs. So far, the process runs smoothly until I attempt to trypsinize the cells, I attempt to...
22 February 2024 9,190 0 View
Is 1mL/kg CCl4 the concentration of the olive oil mixture or the concentration of CCl4 itself to induce liver fibrosis?
14 February 2024 1,536 1 View
Dear researchers, what could be the formation mechanism of the structure that is tiny in size at the end of the first day in the electroless nickel plating waste solution, grows like a mushroom...
14 February 2024 4,569 4 View
Hi all, I am performing an EMSA assay for RNA-Protein binding and I am using SYBR Safe dye. I have 0.75% agaros gel that contain 1X THE buffer and 100mM NaOAC. As you can see, I have lots of...
13 February 2024 4,417 4 View
Can it be that in the primeval ocean, thanks to some energy source like sunlight, volcano or lighting, the amino acids randomly unite a long chain (protein?) and some of which were able to...
13 February 2024 5,584 4 View
specially when the expected gravity sources are more probably occur within the topographic range (between sea level and surface level, I speak about area with average elevation 500m above mean sea...
09 February 2024 7,329 0 View
Why metal ions with high half potential can't be detected on a bare electrode even though corrosion doesn't happen and I'm using the suitable carbon working electrode and Ag/AgCl for the reference...
01 February 2024 301 1 View
I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...
01 February 2024 1,827 1 View
Hi all, I have a set of SNP and SilicoDArT marker data. The SNP points of SNP markers were already provided by the GBS company (Such as G>T). However, the SNP change points (allele change, such...
01 February 2024 5,802 0 View
I have been trying to dock compounds consisting silicon(Ex-Octaphenylsilsesquioxane) with a protein but Pyrx throws up an error saying "Si" is not a valid Autodock type. Note that the Autodock...
30 January 2024 1,966 2 View
Especially since the 1000 Genomes project identified possible ancestral markers.
27 January 2024 8,145 1 View
Hello everyone, After exposing HeLa cells to phthalate, I will detect various antioxidant levels with an ELISA kit. Before ELISA, I will determine the protein concentration of the cells with BCA...
26 January 2024 714 1 View
I am using cobalt column for purification. If anyone has done it, kindly share the protocol.
25 January 2024 2,908 0 View
Hi, I need an exponential equation with two time constants to fit a sodium current tracing. You can find two attached images: the equation I need is Two exponentials and the red tracing which is...
25 January 2024 3,648 1 View
Hi, I am attempting to co-express GFP using a bicistronic vector that contains the T2A peptide. (CD8 alpha signal peptide - GOI - T2A - GFP) I am concerned that, since I am using the CD8a signal...
13 January 2024 8,907 2 View
I am looking for documents, articles etc. on the international/European/Italian nursery sector in terms of supply/demand. I am also looking for information on the relationship between nursery...
07 January 2024 3,049 0 View