The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
Hi, I wanted to ask two questions that would save me a lot of work. Is there a specific function that gives me the set of PDB codes for single point mutations in the sequence of a specific...
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I'm trying to study the function of a gene on cancer cells and would like to study the gene knockdown/knockout model. I found various ways to do study the model but have difficulty choosing the...
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I’m trying to transiently express 75kDa protein in mammalian cells (HeLa) using pCDNA3(cmv promoter). The western blotting result showed in addition to the full length protein other truncated...
26 January 2022 2,173 2 View
I'm purifying a protein which is homotetrameric in nature and each subunit is catalytically active. However, I am a bit uncertain about the molecular weight which I should consider upon...
26 January 2022 9,852 1 View
I am about to use sequence a set of genes in human DNA samples using ion torrent and Illumina to find variants. Do I need a positive control ? to make sure that the sequences were sequenced...
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Hi, I've been trying to transiently transfect RPE-1 cells with midi-prepped plasmids using invitrogen lipofectamine 3000 but can't get to 20%-30% efficiency. No significant cells death issue,...
24 January 2022 4,656 0 View
I'ld like to know what is the difference between ExpiCHO and CHO-S cell line ?
22 January 2022 3,920 3 View
Hello, I'm a medicine student and i'm investigating the effects of a single nucleotide polymorphism on the expression of a phenotype (cuantitative). I have already performed an ANOVA test to...
22 January 2022 6,933 4 View
I do not have a nucleofactor available and I am trying to get it with Gene Pulser. But maybe I am having issues to get the right setting on the equipment or it just really wont work... Was anyone...
21 January 2022 1,185 1 View
Hi! I thought it had always been accepted knowledge in the labs I've worked in that for a given promoter/protein pair, that IN GENERAL, you will get higher expression with transfection than with...
19 January 2022 8,282 3 View
Hi everyone! I am doing some enzymatic with exogenous enzymes in bacteria, and I want to do a B-Glucanase test. The protocol I found so far contains Lichenan, and I wonder if there are other...
18 January 2022 7,295 1 View
When the ligated vector was transfected into E. coli, only a few colonies were grown, and there were no bands in PCR, both times.
14 January 2022 3,881 2 View
I would like to transfect my cells with a plasmid for a fluorescent protein (classical membrane marker I normally transfect via electroporation) AND transfect a siRNA to silence another protein....
13 January 2022 3,438 8 View
Since I have to transfect adherens cells with siRNA by using INTERFERin (never used before), I would like to ask you if anyone has a good protocol for siRNA transfection using INTERFERin. Thank...
13 January 2022 9,476 3 View
HEK293T cells were used as the lentivirus stable transfection packaging cells. After transfected my target cells with the lentivirus (included puromycin resistance) produced, I got a colony of...
13 January 2022 2,320 3 View
Hello, I am trying to express a single domain soluble protein with an N-terminal SUMO tag in E coli. As an alternative, I would like to express this fusion protein in mammalian cells. This will...
11 January 2022 1,222 0 View
I tried docking same ligand with the same protein prepared from different pdb complex from rcsb page. They yielded different results. that made me wonder which pdb complex of the protein should I...
06 January 2022 341 7 View
I am planning to do some research according to an experiment protocol, which needs E. Coli NC11. But I cannot find where to buy it. Did anyone have any ideas on this?
05 January 2022 7,264 4 View
We want to inhibit gene expression via shRNA in prostate cancer cell lines and then store them for a while for further experiments (apoptosis, proliferation, qRTPCR etc.). I know that it is...
05 January 2022 5,701 2 View
Upon transfecting A549 cells with siRNA targeting my gene, the cells acquire a globular(round) shape instead of their fibroblastic phenotype. Gene knockdown has been verified. and the gene has...
04 January 2022 9,316 3 View
I need to disolve tamoxifen citrate tablets without using ethanol, is it possible to use corn oil or DMSO like with tamoxifen.
04 January 2022 7,670 4 View
I have done western blot of mesenchymal stem cells tp check the level of caspase 3 and cleaved caspase 3 in my cells showing apoptosis. interestingly I have increase level of pro caspase 3 as...
30 December 2021 2,279 2 View
Can anyone please share the protocol of live virus production in Hela cells by using infectious viral plasmid?
30 December 2021 4,249 3 View
I have grown 22Rv1 cells for virus transfection. After i added the virus the cells were clean but when i added puromycin for selection of stable cells then after 48 hours these black thread like...
28 December 2021 2,703 3 View