Contact experts in Transgenesis to get answers
812 views 40 posts
Questions related to Transgenesis
I have a construct, HyPer-mito, from the company Evrogen that I am trying to clone and use for transgenesis in zebrafish. The injected stock plasmid downstream of a cmv promoter seems to work;...
02 February 2020 6,288 4 View
I currently am attempting to insert a new promoter sequence ahead of my gene of interest using the Gateway cloning method (Tol2Kit: The Tol2kit: A multisite gateway-based construction Kit for ......
06 June 2019 2,217 3 View
I generated several transgenic Arabidopsis lines that contain a T-DNA in which two transgenes (Gene A and Gene B: see attached figure for more details) are linked. Each transgene has its own...
01 January 2018 5,375 13 View
Current statement concerning plant transgenesis research in Europe?
12 December 2017 1,486 3 View
Hi! I would like to know which are the target sites for the PhiC31 Integrase. So far, I couldn't find any precise information about the Orientation of the attB-Sequence and at which position de...
09 September 2017 4,434 1 View
I have a client that is thinking about using tagBFP2 and they are wondering how well it works in mosquitoes. Has any one used it in mosquitoes what was their experience?
08 August 2017 6,797 0 View
I want to over-express various genes in human ES and iPS cells, mainly snoRNAs and the expression to be stable both in the pluripotent cells and when I differentiate them into the 3 embryonic germ...
11 November 2016 9,226 3 View
Hello, I have experience producing transgenic zebrafish with CRISPR and the CAS9 mRNA would be polyadenilated with a Poly(A) tailing kit after in vitro transcription. However, with Tol2, the...
08 August 2016 469 2 View
My MHC-I transgene under MHC-I gene promoter, is either on or off per cell, leading to a positive and negative population in body, which is different in every mouse. This expression is unanimous...
06 June 2016 3,549 8 View
06 June 2016 4,427 7 View
I am performing transgenesis in zebafish, using a Tol2 system. I have successfully constructed the entry plasmid and the integration of my wished construct has also been confirmed. However, I do...
29 April 2016 5,924 8 View
04 April 2016 8,865 9 View
I need answer as i have to present a topic on recent advances of Genetic engineering for the production of fruit/crops (bio-safety prospective).
02 February 2016 7,029 3 View
Hello, as a behest for the university we have to create a transgenic knock in mouse with a specific deletion of lysine 210 in the TNNT2 gene, which causes the development of dilatated...
10 October 2015 6,893 7 View
05 October 2015 3,927 6 View
Hello, I am trying to knock-in a gene in the safe harbour region AAVS1 using CRISPR, to correct a loss-of-function mutation of my gene of interest. Could an already spliced mRNA sequence of my GOI...
09 September 2015 8,213 4 View
Hello, I am trying to use CRISPR/Cas9 for the knock-in of a transgene using HDR by targeting the safe harbour locus AAVS1 in human iPSC.Is orientation of the transgene important for its...
09 September 2015 6,964 3 View
I want to overexpress some genes in Lactarius volemus, but I do not know the protocols of tissue culture. Can someone provide me with them? Thanks!
02 February 2015 7,970 0 View
Hi, we just planned to make a KO mouse line for a protein we're studying. The ideal would be a conditional and inducible one. Has anyone experience with that? Would that be feasible at all with...
02 February 2015 4,060 5 View
zebrafish transgenesis, Gal4/UAS expression system
11 November 2014 4,407 7 View
Gal4 zebrafish transgenesis. How to identify germline carriers.
10 October 2014 6,241 3 View
Has anyone used the FUW-tetO system for doxycycline-induced transgene expression? I transduced my cells with trangenes (MOI=5 each) under the FUW-tetO, then treated with 1 ug/mL doxycycline for 5...
10 October 2014 2,106 7 View
How can I create resistant TYLCV , what genes are functioning in this process?
09 September 2014 6,274 3 View
01 September 2014 3,723 3 View