The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
I am trying immunofluorescent staining of mouse brain tissues for CD4 and CD8. Does anyone here know any antibody that worked for them in fresh frozen mouse brain tissue? Few information: 1....
01 June 2022 5,703 0 View
There is abundant literature regarding Jurkat T cell activation markers such as CD69, CD62L, and CD107a. However, there are hardly any for deactivation. I can't find whether CD69 downregulates...
24 May 2022 1,250 0 View
Hello everyone I have a question that how to resuspend the 20ug of lyophillized esirna or how to make stock. A detailed protocol such as how to make the stocks what should be stock concentration...
23 May 2022 9,645 0 View
I did an experiment which involved adding DHA to a cancer cell line. Most research suggests that the cells should be affected by its addition at the concentrations I used. However the cells did...
20 May 2022 1,336 2 View
I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using...
13 May 2022 758 1 View
I just needed for my Gaba analysis. Thank you so much po 🥰
05 May 2022 9,586 0 View
I unfortunately do not have a BDNF control. This is mouse hippocampal lysate, with polyclonal rabbit bdnf antibody 1:1000 and goat anti-rabbit IRDye 800 secondary. Chameleon duo ladder and PVDF...
19 April 2022 3,591 5 View
I thawed a tube of Calu-3 cells from ATCC, following product instructions. The flask I used was not coated with any substrate. I used EMEM with 10% FBS and placed it in the usual 37degC incubator...
19 April 2022 4,396 3 View
The company provided only one 96 well plate in the kit, which I have used but the reagents in the kit still remains. I am now thinking of using another 96 well plate different from the one the...
12 April 2022 2,627 5 View
Hi, I want to check interaction strength between my protein of interest and several candidate interactors using Y2H (TakaraBio, previously clontech). If I follow manufacturer's instructions which...
12 April 2022 7,824 4 View
Dear Experts, We would like to inquire if planned to buy commercialized Anti-HER2/neu (369-377) T Cells to perform a cytotoxic T Lymphocyte (CTL) assay. Do we need to use APC to activate T cells...
07 April 2022 6,931 1 View
I want to test my algorithm in some real TCSPC FLIM data. But I cannot find any data available online.
07 April 2022 3,483 1 View
I am looking for a method to purify protein hydrolysates from impurities. I have used enzymes to hydrolyzed ultrasound pretreated oat protein. Initially, the protein content of isolates was about...
07 April 2022 3,904 0 View
I am trying to separate VP1, 2 and 3 using bioanalyzer 2100 instrument. The instrument method is overdiluting the samples. Any one has tried developing separation method for AAV?
05 April 2022 7,960 0 View
Hi, I'm having some trouble running a basic immunoprecipitation experiment. I'm trying to pull-down actin from RAW cells, but all I get is a smear throughout the entire lane with no visible or...
05 April 2022 2,138 9 View
Hello everyone, I am an R beginner and I would like to do a Fisher's exact test 2x2 for each gene on my list (see attached file) to statistically check if there are any differences in...
04 April 2022 7,012 23 View
I did ApoTox-Glo Triplex Assay to asses viability/cytotoxicity and apoptosis on three prostate cancer cell lines (LNCaP, 22Rv1 an PC-3) stimulated with high concentration of docetaxel and...
03 April 2022 1,106 3 View
Hello, I am Mahmuda, now I am working with DG44 cell culture. So far, my cell culture viability improved to 90%. However, before transfection, my cell viability decreased to 70%. I am very...
30 March 2022 498 1 View
Hello, I am Mahmuda, now I do ExpiCHO-S cell culture. As suspension cells, these cell cultures are very susceptible to clumping. This clumping really made my cell culture unhealthy. Are there any...
30 March 2022 5,576 2 View
I would like to learn my students how to perform a co-immunoprecipitation experiment, coupled to western blot of primary antigen and the co-precipitant. Does anyone have a good suggestion for...
28 March 2022 7,149 3 View
Hello everyone, I wanted to ask if anyone has been able to successfully transfect HEK293 cells with the BioRad Gene Pulser XCell and the pre-set settings used for it. I've often ended up with...
28 March 2022 8,160 1 View
I used shRNA plasmid for the knockdown of gene. Firstly, package the virus, then transfect the target cells and then puromycin selection to get the stable cells. After selection of stable cells i...
28 March 2022 7,704 1 View
In my experiments, I found that different monoclonal cell lines from the same host cell produce similar concentrations of ammonium ion when using the same medium; but when the same monoclonal cell...
27 March 2022 4,581 1 View
Hello, I am trying to measure caspase activity in CHO cell samples using the Flurochrome-labeled inhibitor of caspases assay (FLICA). I want to look at ethanol (or another checmical) induced...
25 March 2022 5,823 2 View