The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
I've been trying for several weeks to get consistent and successful transient transfection of glosensor DNA (promega) into Sw620 and Sw480 colon cancer cells in order to measure cAMP levels by...
23 November 2021 8,182 3 View
I want to ask does EGM 2 basal media can be used without supplements to study HUVEC proliferation or requires the addition of the supplement.
23 November 2021 8,573 2 View
Forty-eight hours after si-RNA transfection, RT-PCR and WB showed that the protein has interfered. Now I want to conduct dosing treatment based on the knockout of the target protein to understand...
22 November 2021 1,766 3 View
Hello, Are any of you working on episomal MARS vectors? I want to know how much time / generations they last WITHOUT an initial selection in mammalian cells (any type would do). I could clone...
20 November 2021 887 0 View
I've been working on making a working gateway plasmid with LR clonase. I've been following the protocol step by step, but I don't get colonies. I use the LR clonase II plus from Thermofisher. The...
19 November 2021 3,689 2 View
Dear AAV-community, I am looking for data on excretion kinetics of AAV9 viral vectors from mice. So, I need the information how long urine and feces contain infectious AAVs after i.v....
16 November 2021 1,224 1 View
I am planning to do siRNA transfections and then measure subsequently levels of certain hypothesised downstream genes. Is it necessary to measure cell viabilities in this case as anyways one would...
14 November 2021 1,743 3 View
I transfected my cells (primary human dermal fibroblasts) with a plasmid containing the gene of interest and a C-terminal turboGFP tag. I could see the turboGFP fluorescence using a fluorescence...
12 November 2021 7,059 3 View
In most of the commercial kits, they have a proprietary formulation which is not disclosed.
11 November 2021 269 4 View
I am using G418 to select for stably transduced 22Rv1-luciferase expressing cells. Would anyone know of the minimal effective concentration of G418 required to kill 22Rv1 parental cells? I am...
11 November 2021 8,443 3 View
Hi there! I've used transfection reagents for different experiments over the years, and I'm curious if there's a reasoning to use JetPrime vs PEI vs Lipofectamine? Do any of a particular benefit...
10 November 2021 5,377 3 View
I am performing a luciferase transfection in U87 cell lines to express the luciferin gene. I cultured them in a 24-well plate, and pulled A+B to 1 10-cm dish, then pulled C+D to 1 10-cm dish. My...
09 November 2021 8,355 3 View
Polyethylenimine (PEI) is a polymer used for transfection
08 November 2021 2,375 3 View
Hi everyone, I have a question about the transfection of my RAW264.7 cells. I have produced GFP fusions with my protein of interest (POI) on both the C- and N-terminal side. I have validated these...
04 November 2021 1,701 1 View
I am looking for an HA-tag antibody to use with yeast. The HA-tagged proteins I am trying to assay via WB are endogenously tagged with 3xHA. I don't see very high levels of expression. Therefore,...
04 November 2021 4,615 7 View
Hi I am patching CHO transfected cells with Na 1.2 channels. the biggest issue is the efficacy of transients transfection. The protocol sometimes does not show good efficsy and channels...
28 October 2021 1,609 2 View
I have done steered MD of RNA and now trying to figure out the analysis of the data obtained by the simulation.. I wanted to know on how to plot the force vs time plot and force-extension plot...
27 October 2021 4,935 2 View
I have two treatments; resistant and susceptible. I have to use them in control experiments with replications. How I can use iTRAQ based different labelling to those different treatments’s...
25 October 2021 5,273 3 View
Dear Scientists, a good and efficent strategy for transfecting primary cells would be very helpful... Can someone help? :-) Thanks in advance Bodo
23 October 2021 901 3 View
I've found a few articles describing differentiation protocols from hematopoietic to myeloid linaje for human cells (like the described in Hematopoietic differentiation and production of mature...
20 October 2021 2,125 1 View
When we perform housekeeping genes (such as GADPH) for baseline comparison control in RT-PCR analysis, the degrees of CT values were found to vary a lot between different animals (in our case, 5...
20 October 2021 4,207 2 View
Cell surface proteins can be specifically biotinylated, but the enrichment of biotinylated proteins is based on streptavidin beads that are used upon cell lysis and release of proteins to a...
18 October 2021 5,650 6 View
How long does it take for the exosome to enter the cells? Does the exosome have an ability to express proteins? or are they just carrying them?
14 October 2021 4,102 4 View
Hello every one I'm trying to quantitate essential amino acids in serum or DBS by Lc-MSMS(Quadrupole ABsciex 4500 ) . by searching the net for trusted protocol , all protocols that published...
14 October 2021 6,876 1 View