The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
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05 April 2023 8,639 1 View
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03 April 2023 1,634 0 View
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30 March 2023 2,295 2 View
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28 March 2023 6,645 3 View
In the latest edition (2016) of the Basic Practical Microbiology Manual from the Microbiology Society, all the aseptic procedures are carried on near a Bunsen burner. This is the common practice...
28 March 2023 4,841 6 View
I have a list of identifiers from my IP samples (protein-tag and only tag expressing). I am trying to filter data of common identifiers in both samples. What parameter from the data that I should...
23 March 2023 3,598 0 View
I need to induce epilepsy in rat same of human epilepsy
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what are the advantages of each? and which one is more accurate?
14 March 2023 4,172 2 View
Hi, I am encountering a problem with protein expression and than detecting on western blot. I have around 120kDa membrane bound protein which is now fuse to GFP(~150kDa) to help in selecting...
14 March 2023 6,116 1 View
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13 March 2023 5,978 0 View
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09 March 2023 1,976 2 View
I am currently trying to calculate the vibrational density of states as well as vibrational spectra for an optimized geometry using VASP, but am still new to the program. I have successfully...
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Good day! I have used QuPath as the software for analyzing IHC data and then exported the excel sheet of measurement of annotation with the following columns. Image, Name, Class Parent, ROI,...
01 March 2023 6,176 0 View
Now ,I can get ubiquitin(UB) by Vector pQE-30.How can I get phosphoribosyl ubiquitin(PR-UB)? Need protocol, plz, thanks.
28 February 2023 6,814 0 View
Hi all, Sometimes, transient expression using PEI system needs filler DNA or Boost DNA, but I can not find any paper describing transient expression using lipofectamine system needs filler DNA or...
24 February 2023 4,706 1 View
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17 February 2023 8,863 3 View
I am struggling with determining the right gene isoform of human CD28 and IL-15 to build a published CAR construct for CAR-T or CAR-NK cells. Since the authors did not elaborate on how to select...
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15 February 2023 9,286 0 View
I have prepared 10mM uric acid in borate buffer of pH 8.5 to study the uricase assay as per previous literature. However, the uric acid exhibited autolysis with borate buffer and tris buffer...
09 February 2023 7,302 0 View