The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
I have been trying to transfect MIA PACA 2 using lipofectamine 2000. However, the real time PCR results (Ct values) are the same for the control and the experiment.
24 February 2022 666 3 View
I'm trying to grow Sulfolobus on 6 well plates for my experiment, the plate has 5ml of solid media and I inoculated 300ul of cell culture on them. I'm incubating the plates in a box with soaked...
24 February 2022 8,454 1 View
Recently, we started using the Neon Transfection System in our lab. However, a very confusing concern popped up, and I would really appreciate your kind advice a lot. It is about the decision to...
22 February 2022 6,011 1 View
Hi all I did transfection in 96 well plates and then fixed it with ice-cold 50%acetone and methanol, but could not proceed with immunofluorescence staining the wells for four days. Would it be...
22 February 2022 6,631 3 View
Hi I want to calculate the relative risk of SNPs. I have applied an additive model to check the association of the allele with the disease. can someone guide me on how to get relative risk value...
22 February 2022 3,460 0 View
I Tried doing with lipofectamine RNAiMAX but I could not see much fold change.
17 February 2022 8,543 1 View
Hello all, I am using HEK293T and HEK293-Peak Rapid cells, 0.01% PLL, Tryple Express, Optimem with 4% FBS, Lipofectamine 2000, and plasmid on 96-well plates for a transfection protocol leading...
17 February 2022 7,811 4 View
Dear those who have experience on ARPE-19 cell experiment, Hi, I have been culturing ARPE-19 cells. However, when I put the cells and the media in an incubator, something were observed in the...
17 February 2022 3,955 3 View
I am developing stably transduced HEK293T cells (lentivirus) with constitutively active RhoA gtpase. I have no experience in Microscopy and I want to characterize these cells visually and expand...
16 February 2022 4,277 4 View
I need to find a Mammalian cell line that can translate mRNA of a bacterial gene
16 February 2022 5,791 3 View
hey i'm not sure if when i do concatenate alignment for proteins of different species (for phylogenetics) should I use whole protein alignments (i think so) or alignments of protein domains...
16 February 2022 636 5 View
Dear all, I've been struggling with a problem regarding lentivirus production for quite some time now and I cannot seem to figure it out. I. The goal of my experiments is to knock-down certain...
14 February 2022 4,836 8 View
I am currently trying to transfect EMT6 cells using Neon Transfection. A majority of the cells die within 24 hours of transfection, and the remaining cells show very low expression of GFP. I have...
10 February 2022 3,091 1 View
Hi, I'm kinda new about handling cells and I'm struggling with contamination lately. I use HCT116 and they look fine when I culture them in DMEM + 10% FBS + 1% P/S (the first picture). When I...
10 February 2022 4,818 2 View
Can anyone provide the recipe in dissolving Bovine Serum Albumin in a buffered solution which can be used for cell sorting? I came across the solution: HBSS-BSA-EDTA medium but i'm a little...
09 February 2022 7,490 2 View
Hello, I have been trying to transfect Cancer Associated Fibroblasts (CAFs) with a plasmid of interest. I have tried Lipofectamine, Lipofectamine 3000, Fugene HD, and TransitX2. All of these...
08 February 2022 2,315 5 View
I am trying to use DNA FISH to detect plasmid DNA after transfection. I am curious if one should expect to see signal in the cytoplasm only or cytoplasm and nuclei? Any literature suggestion is...
08 February 2022 3,015 3 View
I am trying to adapt the protocol found below, but they use THP1 cells, I am using murine BMDM. I am wondering if this is possible? I cannot find purified mouse HDL or apolipoprotein anywhere and...
08 February 2022 1,379 2 View
I just joined as a new faculty member and this place uses Bio-Rad systems and I was used to using Invitrogen system earlier and looks like Bio-Rad doesn't have a bi-tris precast mini gel, which I...
08 February 2022 1,598 1 View
I've transfected fibroblasts with GFP tagging mitochondria and I collected the extracellular vesicles secreted . usually with my non transfcted cells, I used to see mitochondrial material in my...
04 February 2022 2,193 3 View
Has someone experience with plasmid transfection of DU145 prostate cancer cells for a transient transfection? What reagent/method can you recommend? Fugene, Lipofectamine 2000/3000,...
02 February 2022 7,246 2 View
I seem to be struggling to transfect plasmid into KBM-7s with numerous common transfection reagents I have tried! Has anyone successfully transfected them? All of my control cells are happily...
02 February 2022 934 1 View
Hi, I wanted to ask two questions that would save me a lot of work. Is there a specific function that gives me the set of PDB codes for single point mutations in the sequence of a specific...
01 February 2022 9,675 1 View
I'm trying to study the function of a gene on cancer cells and would like to study the gene knockdown/knockout model. I found various ways to do study the model but have difficulty choosing the...
01 February 2022 3,520 4 View