The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
2,330 views 4,037 posts
Questions related to Transfection
I have a U937 monocyte cell line at passage 23 in culture, but it is growing very slowly, and the overall culture appears weak with few cells. I have been culturing them in 20% fetal bovine serum...
11 September 2024 2,601 2 View
Greetings, I need to identify bacteria of the genus Bacillus (B. licheniformis, B. subtilis, B. megaterium). For this, I want to use API 50 strips. I don't understand what equipment I need for...
11 September 2024 2,351 0 View
I need to do treatment of Mtt assay for 24, 48 and 72 hours treatment of my compound. First method, Monday - seed the cells Tuesday- treatment for 72 hours wednesday - treatment for 48 hours...
09 September 2024 8,685 2 View
كيف يمكن أن تكون المعرفة طريقا إلى السعادة؟
08 September 2024 9,118 3 View
Dear friends, I need to transform my qPCR data into a heatmap to make it more visualized. I have seen this form in several articles, but I'm not sure about some details: 1. Should the P value be...
07 September 2024 3,987 0 View
I am looking for a protocol for bactearial decontamination in cell culture. Looks like bacillus for me. Is there any reference for that? There is no mycoplasma.
06 September 2024 1,350 3 View
Hi everyone, I'm currently running RT-qPCR on my cells (with Sh-Scramble and Sh conditions). Previously, the Ct values for my Sh-Scramble cells were consistently in the range of 24-25.8 across...
05 September 2024 3,315 1 View
Dear all, I have installed GPU GROMACS 2023 on two PCs, and one is working without errors. However, the other one gives a segmentation fault (core dumped) error during the gmx run. I launched...
05 September 2024 2,158 2 View
I am planning to use Cycloheximide (CHX) chase assay to determine protein stability in myoblast cell line. I am going to perform this experiment for the first time so I am really confused about...
04 September 2024 1,068 0 View
Hello, I recently performed an apoptosis analysis using the MUSE Cell Analyzer with the Annexin V-PE and 7-AAD kit. After 24 hours of treatment, my results indicated approximately 20% of cells in...
28 August 2024 2,324 2 View
I need to perform yeast cell transformation after Golden Gate assembly in a 96 well plate. The cassette will be excised from the vector by RE digestion before transformation and usually, we clean...
28 August 2024 9,574 5 View
Hi there fellow respected researchers and industry players. I am currently facing a problem with isolating mesenchymal stem cells from menstrual blood. To address the concern of contamination from...
20 August 2024 8,939 5 View
Hello, I am Varshini Selvakumar pursuing MSc in Clinical Psychology in Bangalore, India. I am currently working on my research on " Personality traits and tolerance of ambiguity-Mediating role of...
12 August 2024 5,343 1 View
I carried out MMPBSA calculation using gmx MMPBSA module and allowed for entropy calculation through mmbsa.in the parameter file. it yielded two values 1- interaction entropy approximation 2-c2...
12 August 2024 8,936 0 View
In QM wavefunctions are written down as it’s square is the probability distribution where you find the particle in the space. Is it possible to flip the space axis to time axis and this way you...
08 August 2024 180 4 View
Hi everyone. I am trying to perform the selection process of the transfected GS-deficient CHO cells with MSX, I use 50 uM MSX which should be more than enough but cells have still above 90%...
08 August 2024 4,784 1 View
Hello! I isolated mesenchymal stem cells from mouse bone marrow. They have been there for 19 hours and they did adhere but they are still spheroids. Did they no longer survive?
06 August 2024 1,536 1 View
The organism used in the biofilm inhibition assay is Staphylococcus aureus. While performing the experiment in microtitre plate, I used 200 microlitres of (1:100 dilution of culture broth with...
03 August 2024 1,767 1 View
I recently switched over from a windows laptop to a macbook pro m3 and wanted to run a phenogramic analysis on the some protein sequences. I discovered that my I was unable to install RAxML (the...
31 July 2024 239 1 View
Hi folks. I am looking for alternative ways to study water distribution and mobility in my food sample. Typically time-domain NMR is employed to identify the T2 relaxation time corresponding to...
30 July 2024 5,402 2 View
Ayahuasca is an Amazonian brew, used by mestizos and Indigenous communities to have visions and understand their feelings and thoughts. There is a potential therapeutic effect, but there isn't...
29 July 2024 8,835 2 View
Dear Researchers, As a Guest Editor for JoVE, I am organizing a Methods Collection on ‘Harnessing the Power of Electroless Plating for Sustainable Materials in Advanced Energy Storage...
26 July 2024 5,371 0 View
Hello, I have rheometry measurements done at a collaborators' lab with a Haake Mars rheometer, and I would like to know if there is a software (free) I could use to open/export these files,...
17 July 2024 5,646 0 View
I want to find out the number of layers in the two-dimensional material by using the wavelength of the exciton in the absorption spectrum.
11 July 2024 9,761 0 View