The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
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A published paper mentioned that DOTAP liposomal transfection reagent could deliver RNA to endosomes. Does lipo 3000, lipo 2000, RNAiMax can achieve the same results? Thanks.
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What impact do different promoters such as CMV, CAG, CAAG, etc., have on the efficiency of viral transductions? My observations are, NOT THAT MUCH! Any inputs?
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I have two groups of cells. Group A showed lower proliferation and migration compared to group B. The cell cycle analysis of the two groups showed a larger G0/G1 phase and smaller G2/M for group...
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The antibiotics are Neomycin, Puromycin, Zeocin, Blasticidin
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Hello everyone. I have questions regarding comparing frequencies between groups. I will be happy if someone can help. So I will describe first briefly my research design: - I am analysing online...
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I am preparing for a proliferation assay where I have infected KP230 cells with shScr, shCol18a1#35, and shCol18a1#37 viruses. These shRNA viruses are made with the pLKO.1 vector and have...
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My lab usually grows HEK293FT cells in an adherent monolayer culture. However, I wanted to try and increase our recombinant protein yield by growing the cells in suspension. I think this is...
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Hello, I work on the differentiation of stem cells into cardiomyocytes. For this I use the PSC Cardiomyocyte Differentiation Kit (ThermoFisher). After differentiation (day 14) I can't dissociate...
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Hi everyone I prepared a 5mM stock of tamoxifen citrate dissolved in DMSO but when I supply 10uM of it as said in the datasheets, it didn't cause cell death within 48 h. What's the problem?
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Hello, I am trying to find a cheaper replacement for the item below. I've found a few options but i'm confused why some say 154 aa or 147 aa. I understand that the aa stands for amino acids but...
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We are planning to perform pull-down assay, but I am a new in that field. Which tag for affinity purification of the “bait ” protein is better? is there really significant differences between GST...
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Hi When running clade model c in PAML, one branch that I think may experience elevated dN/dS is put in the foreground by me. After the run, the foreground has a dN/dS = 0.65 while the background...
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For the past few weeks we've been using three different cell types on the Fura 2 calcium experiment including iPSC's, RenCells, and human skeletal muscle cells. We loaded our cells with 2.5 uM of...
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Particularly wrt spatial cognition and navigation. It is well know that the right MTL/hippocampal formation is highly involved in spatial information processing but in some special cases, the left...
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Hi, Any idea on how to reduce variations among triplicates during assay such as LDH?
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Dear all, Which is the best method to isolate tumour organoids from methacrylated collagen hydrogel. I need to isolate RNA from hydrogel cultured tumour organoids. Thank you for your help!
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