The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
I am trying to transfect miRNA into buffalo endometrium epithelium cells with Lipofectamine RNAiMAX. To check miRNA transfection efficiency manufacturer's protocol has suggested using a KIF11...
13 October 2021 5,384 2 View
Hi all, I am conducting an ELISA assay to determine the concentration of recombinant protein in the cell culture supernatant after transfection. I have many different transfection conditions, with...
13 October 2021 4,593 3 View
Hello, i am currently trying to overexpress the CSF-1R receptor in the THP-1 cells in order to create a cell line to be used for the testing of CSF-1R inhibitors. In order to achieve this i am...
11 October 2021 5,771 2 View
I recently started to do CRISPR in HeLa cells, tagging a GFP to my target protein. In principle, I need to co-transfected HeLa cells with two plasmids. One contains the gRNA-Cas9 and the other one...
06 October 2021 3,157 2 View
I recovered the IPS line three days ago according to a standard protocol, however, there seems to be necrosis in the clones. Things get worse today. I use the mTeSR plus and change the spent media...
05 October 2021 425 4 View
Hello, I am studying T-Cell activation and while we have markers for T-Cell activation that work reliably well we are looking for the population of T-Cells that were not activated despite being...
04 October 2021 4,296 2 View
I'm looking for a protocol to transfect my cells with luciferase, can anyone help me? Thanks a lot!
03 October 2021 3,931 1 View
Does anyone have experience with siRNA transfection with the Neon on THP1 cells? can anyone share the parameters and when do you add the PMA?
01 October 2021 4,623 3 View
Hello, I am searching for a non cancerous human cell line, poorly tranformed or immortalized that can be well transfected and have a good proliferation rate. Any suggestions? Thank you
30 September 2021 5,442 6 View
Hello friends! We are trying to measure the half-life of transfected IVT mRNA by qPCR. However, we are having trouble completely removing uninternalized mRNA from the media during lysis, which...
26 September 2021 5,629 2 View
I added 3 µg plasmid DNA containing the GFP gene to 75 µL optimem and I added 7,5 µL lipofectamine 2000 reagent to 75 µL optimem. I've incubated the plasmid DNA with lipofectamine and optimem at...
24 September 2021 5,244 3 View
Hello everybody, I am trying to do some tests to improve the adhesion of HEK 293T cells in T-flask, and I wanted to ask you which matrix do you think is the best to grow them in. Thank you very...
23 September 2021 624 2 View
Hi, I transfected HEK cells with fluorescent ABCG2 transporter (multidrug resistance transporter) , it has a GFP probe attached, and I am performing a live cell confocal microscopy experiment,...
22 September 2021 4,653 2 View
Dear all, I have been preparing lentivirus particles (2nd gen) with both transgene of interest, and control lentivirus of GFP only by transfection of HEK293T with FuGENE HD reagent. pVSV-G...
21 September 2021 4,471 6 View
May I measure the CD107a marker on NK cells derived from patients treated with immunotherapy directly without in vitro stimulation with target cells or interleukins? I suppose that expression of...
21 September 2021 6,207 5 View
I want to know what are the different cell lines available for Polycystic ovary syndrome. What cell lines can be best used to understand insulin resistance in PCOS?
15 September 2021 3,117 2 View
It is well known that Mg ions bind PPi moieties (pyrophosphates) during DNA amplification such as PCR, LAMP or RCA. I assume they should do the same during DNA replication in vivo, i.e. inside...
14 September 2021 4,352 1 View
Hi, I have established the best transfection condition that maximizes the silencing effect of Dharmacon siRNA (with Dharmafect-1) on my gene of interest (GOI). My study aims to evaluate the effect...
13 September 2021 2,108 4 View
Pardon me, but it would be great if anyone who had successfully done calcium phosphate transfection on MCF10A cell line can share their protocol/recipes. I would want to compare with mine. I've...
09 September 2021 8,551 1 View
Hello everybody, I am currently doing interaction studies of two proteins which I am overexpressing in HEK293 cells. So far, I have been co-transfecting my cells with two seperate constructs. To...
07 September 2021 4,206 3 View
Dear all, I got these two plasmids from Addgene (#86849 and #138714) and tried to transduce into MDA-MB-231 cell line using the lentivirus. Therefore, I packaged these two plasmids in 293T cells...
06 September 2021 8,722 6 View
Dear all, I am want to generate a k.o. with CRISPR/CAS9 in H292 cells and have issues with the transfections. It does not matte if its for plasmid or RNP format. Does anyone have a working...
03 September 2021 1,321 2 View
I want to propagate Adenoviruses: Ad5 and Ad26 using HEK cells. Among HEK293 and HEK293T, which one is better for the purpose and why?
03 September 2021 5,905 2 View
Hi everyone, I've been looking online for resources to calculate the mass ratio between JetMessenger and my mRNA that I am using for transfection. However, I haven't been getting any relevant...
01 September 2021 5,177 1 View