The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
1,610 views 3,440 posts
Questions related to Transfection
Hello everyone, I am extremely confused about analysis of qPCR data after virus infection or stimulations, and I need help. I hope I can explain properly. Let's say we have two different cell...
30 May 2024 6,321 2 View
In DBS settings, I have seen stimulus widths of up to 500 us in duration; however, the most common pulse duration seems to be between 90-150 us. Clinically, mono- or bi-polar electrodes can be...
27 May 2024 5,783 0 View
We are preparing to submit a manuscript in field of nociceptor/immunity. However looking at our current budget we realize we won't be able to afford the common $2000 processing fees charged by...
24 May 2024 4,654 3 View
I am working with PopArt (Population Analysis with Reticulate Trees) software making statistical parsimony haplotype networks. It uses nexus format, and I had not trouble formatting and making a...
23 May 2024 533 2 View
liposome, essentail oi
20 May 2024 4,861 1 View
I need a bacteriolytic antibiotic for destroy only extracelullar gram negative bacteria Any suggestions?
17 May 2024 7,553 5 View
I am working on corrosion test with an electrochemical jar test. Working electrode: Copper Reference electrode: Ag/AgCl with 3M KCl solution Counter electrode: graphite In the process of linear...
13 May 2024 9,421 1 View
I am facing a loss of viability in lymphocyte cells from human tonsils that I culture ex vivo. I have cultured dozens, but in the last months, small dots always appear in my media and the...
13 May 2024 2,898 3 View
Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...
06 May 2024 3,275 1 View
I need to immobilize several polysaccharides (mainly glucans) on microplate wells for further treatment and analysis by fluorescence. Any suggestions?
02 May 2024 2,174 0 View
I want to perform synthesis of reported compound can you suggest some techniques to find novel compound with better results
02 May 2024 5,884 2 View
can you suggest any schrodinger interface for scaffold hopping
02 May 2024 1,311 0 View
Hello, I am a doctoral student in Brazil. I need to perform the transfer to check for fibronectin immunodetection (250 kDa). Our transfer buffer was heating up too much; I even changed the ice...
26 April 2024 1,668 1 View
presently I'm doing one study on ASSOCIATION OF COMT GENE VARIANT WITH ALCOHOL USE DISORDER. the methodology consists of conventional pcr with comt primers and rflp with NIA III rest enzyme. after...
23 April 2024 4,898 3 View
Hi ! I am trying to run some gels but everytime I do, my samples end up looking very wavy while the scale is ok. For now my protocol is : sds page gel 12% acrylamide, run at 200V at room...
11 April 2024 4,840 4 View
Hi, I observed a strange behaviour in my cell lines I had never seen before. Those are stable lines from Ovarian cancer (line's name: HEY) and from Prostate cancer (line's name: PC-3). Both lines...
09 April 2024 1,383 0 View
Hi, I am for the first time struggling with TRAF2 full length protein purification from BL21 or Gold but I fail to purify it although I tried GST, MBP and His tag it the N- terminal. Do any...
08 April 2024 1,055 0 View
Hi all! I have to quantifiy some proteins in RBCs by ELISA. Since RBC number cannot be standardized and it obviously affect the results, there is the need of some normalization. I read that many...
03 April 2024 3,297 5 View
I’m working on cultivating retinal organoids, but it can’t success form eye field primordial clusters (EFPs) so far. Therefore, I would like to inquire if anyone has encountered a similar issue...
30 March 2024 6,770 0 View
Hello, I do IP using HA-tagged beads. One of my proteins through which I precipitate complex has HA-tag. I see this protein (with HA-tag) after IP. The signal is strong and bright. The complex...
29 March 2024 4,460 2 View
I am a graduate student doing cancer-related research in Korea. When certain drugs were used, cleaved caspase-3 was confirmed as apoptotic appearance was observed morphologically. However, the...
25 March 2024 4,283 0 View
I do not use capacitance compensation at all for sodium current recording in isolated cardiomyocytes. Only series resistance compensation is used. Therefore, my recording starts with a large...
25 March 2024 1,165 0 View
I have sequence of Mitochondrial proteins at which ubiquitination occurs. i want to find out whether its located on MTS or not.
20 March 2024 8,928 0 View
Does phosphorus limit phytoplankton growth and why does phosphorus limit primary production in freshwater lakes?
19 March 2024 1,699 7 View