The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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Questions related to Transfection
Hi, I have biomarker data given in log2-scale, and I am looking into different approaches on how to present the results (differences between two groups). I have seen a paper (with similar data)...
20 September 2022 7,105 4 View
I am considering to combine two different primary antibodies to detect different target proteins on the same membrane (Nitrocellulose/PVDF) for western blot. I found that this works but my...
10 September 2022 4,287 3 View
A published paper mentioned that DOTAP liposomal transfection reagent could deliver RNA to endosomes. Does lipo 3000, lipo 2000, RNAiMax can achieve the same results? Thanks.
03 September 2022 203 1 View
What impact do different promoters such as CMV, CAG, CAAG, etc., have on the efficiency of viral transductions? My observations are, NOT THAT MUCH! Any inputs?
08 August 2022 1,091 4 View
I need to facs sort macrophages, there are many surfaces markers which are used for this purpose such as: CD14, CD68,CD64, CD11c, CD204,CD206. I need to know why certain surface markers are...
05 August 2022 9,236 2 View
I have two groups of cells. Group A showed lower proliferation and migration compared to group B. The cell cycle analysis of the two groups showed a larger G0/G1 phase and smaller G2/M for group...
04 August 2022 710 0 View
Can you suggest some proteins.
30 July 2022 2,594 5 View
Hello everyone. I have questions regarding comparing frequencies between groups. I will be happy if someone can help. So I will describe first briefly my research design: - I am analysing online...
27 July 2022 9,913 3 View
I am preparing for a proliferation assay where I have infected KP230 cells with shScr, shCol18a1#35, and shCol18a1#37 viruses. These shRNA viruses are made with the pLKO.1 vector and have...
22 July 2022 5,950 1 View
My lab usually grows HEK293FT cells in an adherent monolayer culture. However, I wanted to try and increase our recombinant protein yield by growing the cells in suspension. I think this is...
15 July 2022 5,857 0 View
Hello, I am trying to find a cheaper replacement for the item below. I've found a few options but i'm confused why some say 154 aa or 147 aa. I understand that the aa stands for amino acids but...
22 June 2022 134 1 View
We are planning to perform pull-down assay, but I am a new in that field. Which tag for affinity purification of the “bait ” protein is better? is there really significant differences between GST...
22 June 2022 1,838 2 View
Hi When running clade model c in PAML, one branch that I think may experience elevated dN/dS is put in the foreground by me. After the run, the foreground has a dN/dS = 0.65 while the background...
13 June 2022 6,712 1 View
For the past few weeks we've been using three different cell types on the Fura 2 calcium experiment including iPSC's, RenCells, and human skeletal muscle cells. We loaded our cells with 2.5 uM of...
13 June 2022 4,604 1 View
Hi, Any idea on how to reduce variations among triplicates during assay such as LDH?
11 June 2022 9,810 0 View
Dear all, Which is the best method to isolate tumour organoids from methacrylated collagen hydrogel. I need to isolate RNA from hydrogel cultured tumour organoids. Thank you for your help!
09 June 2022 837 3 View
I am trying immunofluorescent staining of mouse brain tissues for CD4 and CD8. Does anyone here know any antibody that worked for them in fresh frozen mouse brain tissue? Few information: 1....
01 June 2022 5,688 0 View
Hello everyone I have a question that how to resuspend the 20ug of lyophillized esirna or how to make stock. A detailed protocol such as how to make the stocks what should be stock concentration...
23 May 2022 9,635 0 View
I unfortunately do not have a BDNF control. This is mouse hippocampal lysate, with polyclonal rabbit bdnf antibody 1:1000 and goat anti-rabbit IRDye 800 secondary. Chameleon duo ladder and PVDF...
19 April 2022 3,579 5 View
I thawed a tube of Calu-3 cells from ATCC, following product instructions. The flask I used was not coated with any substrate. I used EMEM with 10% FBS and placed it in the usual 37degC incubator...
19 April 2022 4,381 3 View
The company provided only one 96 well plate in the kit, which I have used but the reagents in the kit still remains. I am now thinking of using another 96 well plate different from the one the...
12 April 2022 2,616 5 View
Dear Experts, We would like to inquire if planned to buy commercialized Anti-HER2/neu (369-377) T Cells to perform a cytotoxic T Lymphocyte (CTL) assay. Do we need to use APC to activate T cells...
07 April 2022 6,917 1 View
I want to test my algorithm in some real TCSPC FLIM data. But I cannot find any data available online.
07 April 2022 3,452 1 View
I am trying to separate VP1, 2 and 3 using bioanalyzer 2100 instrument. The instrument method is overdiluting the samples. Any one has tried developing separation method for AAV?
05 April 2022 7,950 0 View