The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA. | Contact experts in Transfection to get answers
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I was using the MycoSensor PCR Asssay kit from Agilent (Cat. 302108) and they discontinued it, I would like you to recommend a similar one.
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Dear friends: I am confused about my experiments that have a significant difference between mock control and negative control(scramble).I used lentivirus to infect NK-92 cells, followed by a...
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Hello I am using two expression vectors (pCAGGS and pCDNA) for transforming a fragment of gene (2kbp). For transformation, I am using NEB 10 beta-competent E.coli cells. Issue? Although my...
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Does anyone have any idea where I can find Consumptive Behavior focuses on lifestyle Measurement fully with the blue print, indicator, etc for me to finish my dissertation undergraduate, with the...
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Good day, I tried to install CUDA 12.6 on Linux Mint 2022 and encountered this problem. After command: cmake .. -DGMX_GPU=CUDA -DCUDA_TOOLKIT_ROOT_DIR=/usr/local/cuda I got an output: CMake...
12 October 2024 8,622 4 View
Hi, I want to check the rest amount of mimic miRNA in cell culture after 24 hour-transfection but mimic is RNA duplex. How to reverse transcription with mimic miRNA duplex?
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Dear all, I have been trying to gateway clone two genes, both around 3.2kb in size. However, I am running into a peculiar issue where my colony PCR is positive but once I isolate the plasmid, the...
09 October 2024 3,098 3 View
Revvity biosciences has stoped making the Revvity Health Sciences Inc RediJect Coelenterazine h Bioluminescent Substrate. Has anyone found or used a good substitute that has a short half-life for...
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My system is made of a protein that binds to the membrane, which carries the epitope tag, and a nanobody, binding this tag. Membrane binding protein is a fusion protein consisting of a protein...
08 October 2024 2,400 2 View
I used both lipofectamine RNA iMax and electroporation method and always gene expression level in negative control group is 40% lower than untreated group.
08 October 2024 2,523 3 View
Hi everyone. Our team encountered a problem with colon organoids. We isolate crypts successfully, they look alive (as we see by trypan blue staining), then seed them in Organoid-Star Matrigengel...
02 October 2024 2,912 1 View
I am using a scavangers study. I am facing difficulty for this calculation. Please help me. I have a solution of 100ml TBA. As I understand from youtube, I calculated that I need to take like...
01 October 2024 7,190 3 View
Right triangle hypotenuse is basically a geometric mean of hypotenuse sections generated due to perpendicular line from vortex to hypotenuse. But it's developed using Congruency theory among two...
29 September 2024 3,479 1 View
As part of my research on AI Crime, I am currently working on the problem of deepnude - that is, undressed deepfakes used mainly by young people. The topic seems very interesting to me from a...
28 September 2024 7,203 0 View
Right triangle generally have one angle of 90 degree and the biggest side is its hypotenuse that can be equal to the sum of two other sides known as base Its and its perpendicular respectively.
27 September 2024 9,038 1 View
Hello, I have been trying to detect very low molecular weight proteins (14-17 kDa). I used a 15% polyacrylamide gel and I couldn't see my protein. With a pre-cast gel (4-12% gradient...
26 September 2024 7,856 8 View
I’m running a Real-Time PCR to amplify RNA using primers and a Cy5-labeled probe, but instead of the expected sigmoidal amplification curve, the fluorescence curve declines. What could be causing...
25 September 2024 2,984 4 View
If I wanted to do cell viability using mts assay, the treatment need to be diluted with complete media or basal media? What happen if I used complete media to dilute the treatmemt and do cell...
25 September 2024 1,372 1 View
I found that the solution is light yellow. Is that normal?
24 September 2024 4,992 0 View
To what extent do negative ( or maladaptive ) male student relationships influence decisions to engage in criminal or violent behaviors?
24 September 2024 9,953 1 View
I recently found suspicious big cells in my THP-1 cell culture. It seems like some cells but much larger than the others. What could it be? Is it contamination or cell morphology changes?
21 September 2024 1,201 1 View
I have designed primers for sgRNA for crispr cas9 knockout, my primers doesnt include PAM sequence, but one bp is missing right before the PAM sequence, however the rest of the sequence is...
20 September 2024 4,620 0 View
Hi, I bought 3T3-L1 cells from ATCC and would like to differentiate them into white adipocyte-like phenotypes. I thawed the cells and cultured them in DMEM+10%BCS+1%P/S (37C, 5%CO2). The medium...
17 September 2024 3,575 4 View
Why nobody talks about the momentum mismatch of bulk phonon polariton as they do in surface phonon polariton? Thanks!
15 September 2024 8,927 3 View