I extracted TNA from leaves samples with CTAB-based protocol. Before performing the DNase treatment and getting RNA from my samples, I ran a gel to check the quality of extracted samples: I could see all RNA bands but not DNA bands. Why?
Hi Anna,
this is hard to say without further information (e.g. protocol)
One possibilty would be that if your DNA is not really clean the high molecular genomic DNA remains stuck in the well.
But maybe you can show a picture of the gel to see what is going on.
KR Dörthe
You don't expect to see "bands" from extracted, non-amplified gDNA. At most, you would see a faint, high-molecular weight smear. Unless you are making a DNA library for a next-gen sequencing run (or similar protocol), no one bothers to put gDNA on an agarose gel.
If you want to know the concentration, use a spectrophotometer.
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